Anti-Intersectin 1抗体(ab118262)
Key features and details
- Rabbit polyclonal to Intersectin 1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-Intersectin 1抗体
参阅全部 Intersectin 1 一抗 -
描述
兔多克隆抗体to Intersectin 1 -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Recombinant fragment, corresponding to amino acids 646-840 of Human Intersectin 1 (BC116186).
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阳性对照
- Human fetal brain lysate
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Lyophilized:Reconstitute in 200µl Steriled Distilled Water -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituent: 1% BSA -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab118262于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (1) |
1/500 - 1/1000. Predicted molecular weight: 195 kDa.
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说明 |
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WB
1/500 - 1/1000. Predicted molecular weight: 195 kDa. |
靶标
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功能
Adapter protein that may provide indirect link between the endocytic membrane traffic and the actin assembly machinery. May regulate the formation of clathrin-coated vesicles. Isoform 1 could be involved in brain-specific synaptic vesicle recycling. Inhibits ARHGAP31 activity toward RAC1. -
组织特异性
Isoform 2 is ubiquitous in adult and fetal tissues with high expression in skeletal muscle, heart, spleen, ovary, testis and all fetal tissues tested and low expression in thymus, blood, lung, liver and pancreas. Isoform 1 is expressed almost exclusively in the brain, in all brain regions. Not expressed in the spinal cord. -
序列相似性
Contains 1 C2 domain.
Contains 1 DH (DBL-homology) domain.
Contains 2 EF-hand domains.
Contains 2 EH domains.
Contains 1 PH domain.
Contains 5 SH3 domains. -
结构域
SH3-3, SH3-4 and SH3-5, but not SH3-1 and SH3-2 domains, bind to dynamin (By similarity). SH3-1 and SH3-4 bind to ARHGAP31.
The KLERQ domain binds to SNAP-25 and SNAP-23. -
细胞定位
Endomembrane system. Cell junction > synapse > synaptosome. Cell projection > lamellipodium. - Information by UniProt
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数据库链接
- Entrez Gene: 6453 Human
- Entrez Gene: 16443 Mouse
- Entrez Gene: 29491 Rat
- Omim: 602442 Human
- SwissProt: Q15811 Human
- SwissProt: Q9Z0R4 Mouse
- SwissProt: Q9WVE9 Rat
- Unigene: 160324 Human
see all -
别名
- Human intersectin SH3 domain containing protein SH3P17 antibody
- Intersectin 1 SH3 domain protein antibody
- intersectin 1 short form variant 3 antibody
see all
图片
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab118262 被引用在 6 文献中.
- Catsburg LA et al. Dynamics and nanoscale organization of the postsynaptic endocytic zone at excitatory synapses. Elife 11:N/A (2022). PubMed: 35072626
- Xie C et al. Intersectin 1 (ITSN1) identified by comprehensive bioinformatic analysis and experimental validation as a key candidate biological target in breast cancer. Onco Targets Ther 12:7079-7093 (2019). PubMed: 31564893
- Richter K et al. VGLUT1 Binding to Endophilin or Intersectin1 and Dynamin Phosphorylation in a Diurnal Context. Neuroscience 371:29-37 (2018). PubMed: 29199069
- Ashraf S et al. Mutations in six nephrosis genes delineate a pathogenic pathway amenable to treatment. Nat Commun 9:1960 (2018). PubMed: 29773874
- Hernández-García R et al. Endothelial RhoGEFs: A systematic analysis of their expression profiles in VEGF-stimulated and tumor endothelial cells. Vascul Pharmacol 74:60-72 (2015). PubMed: 26471833
- Humphries AC et al. Cdc42 and the Rho GEF intersectin-1 collaborate with Nck to promote N-WASP-dependent actin polymerisation. J Cell Sci 127:673-85 (2014). PubMed: 24284073