概述

  • 产品名称
    Anti-Intersectin 1抗体
  • 描述
    兔多克隆抗体to Intersectin 1
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Recombinant fragment, corresponding to amino acids 646-840 of Human Intersectin 1 (BC116186).

  • 阳性对照
    • Human fetal brain lysate

性能

应用

Our Abpromise guarantee covers the use of ab118262 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Predicted molecular weight: 195 kDa.

靶标

  • 功能
    Adapter protein that may provide indirect link between the endocytic membrane traffic and the actin assembly machinery. May regulate the formation of clathrin-coated vesicles. Isoform 1 could be involved in brain-specific synaptic vesicle recycling. Inhibits ARHGAP31 activity toward RAC1.
  • 组织特异性
    Isoform 2 is ubiquitous in adult and fetal tissues with high expression in skeletal muscle, heart, spleen, ovary, testis and all fetal tissues tested and low expression in thymus, blood, lung, liver and pancreas. Isoform 1 is expressed almost exclusively in the brain, in all brain regions. Not expressed in the spinal cord.
  • 序列相似性
    Contains 1 C2 domain.
    Contains 1 DH (DBL-homology) domain.
    Contains 2 EF-hand domains.
    Contains 2 EH domains.
    Contains 1 PH domain.
    Contains 5 SH3 domains.
  • 结构域
    SH3-3, SH3-4 and SH3-5, but not SH3-1 and SH3-2 domains, bind to dynamin (By similarity). SH3-1 and SH3-4 bind to ARHGAP31.
    The KLERQ domain binds to SNAP-25 and SNAP-23.
  • 细胞定位
    Endomembrane system. Cell junction > synapse > synaptosome. Cell projection > lamellipodium.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Human intersectin SH3 domain containing protein SH3P17 antibody
    • Intersectin 1 SH3 domain protein antibody
    • intersectin 1 short form variant 3 antibody
    • intersectin 1 short form variant, 11 antibody
    • Intersectin short form antibody
    • intersectin short variant 12 antibody
    • Intersectin-1 antibody
    • Intersectin1 antibody
    • ITSN 1 antibody
    • ITSN antibody
    • itsn1 antibody
    • ITSN1_HUMAN antibody
    • MGC134948 antibody
    • MGC134949 antibody
    • OTTHUMP00000067855 antibody
    • OTTHUMP00000067856 antibody
    • OTTHUMP00000067857 antibody
    • OTTHUMP00000194810 antibody
    • OTTHUMP00000194811 antibody
    • OTTHUMP00000194812 antibody
    • OTTHUMP00000206688 antibody
    • SH3 domain containing protein 1A antibody
    • SH3 domain protein 1A antibody
    • SH3 domain-containing protein 1A antibody
    • SH3D1A antibody
    • SH3P17 antibody
    • Src homology 3 domain containing protein antibody
    see all

图片

  • Anti-Intersectin 1 antibody (ab118262) at 1/500 dilution + Human fetal brain lysate

    Predicted band size : 195 kDa
    Additional bands at : 129 kDa (possible isoform),138 kDa (possible isoform).

实验方案

文献

This product has been referenced in:
  • Humphries AC  et al. Cdc42 and the Rho GEF intersectin-1 collaborate with Nck to promote N-WASP-dependent actin polymerisation. J Cell Sci 127:673-85 (2014). Read more (PubMed: 24284073) »

See 1 Publication for this product

客户评价及客户问答

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Dorsal root ganglion)
Gel Running Conditions
Reduced Denaturing (12.5)
Loading amount
25 µg
Treatment
Mutants
Specification
Dorsal root ganglion
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Anu Shanu

Verified customer

提交于 Mar 24 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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