The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
Use at an assay dependent concentration. ab18404-Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody.
Use at an assay dependent concentration. Ab87303 can be used as a capture antibody in a sandwich ELISA for quantifying mouse IFN-? protein levels. Biotinylated form of the antibody can be paired with a suitable purified capture antibody, with purified recombinant human IFN-? as the standard.
功能Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.
组织特异性Released primarily from activated T lymphocytes.
疾病相关In Caucasians, genetic variation in IFNG is associated with the risk of aplastic anemia (AA) [MIM:609135]. AA is a rare disease in which the reduction of the circulating blood cells results from damage to the stem cell pool in bone marrow. In most patients, the stem cell lesion is caused by an autoimmune attack. T-lymphocytes, activated by an endogenous or exogenous, and most often unknown antigenic stimulus, secrete cytokines, including IFN-gamma, which would in turn be able to suppress hematopoiesis.
序列相似性Belongs to the type II (or gamma) interferon family.
翻译后修饰Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161.
Vikingsson A et al. Altered kinetics of CD4+ T cell proliferation and interferon-gamma production in the absence of CD8+ T lymphocytes in virus-infected beta2-microglobulin-deficient mice. Cell Immunol173:261-8 (1996).
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Hsieh B et al. In vivo cytokine production in murine listeriosis. Evidence for immunoregulation by gamma delta+ T cells. J Immunol156:232-7 (1996).
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