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Recombinant full length protein (Human).
This product was changed from ascites to tissue culture supernatant on 28/11/2017. Please note that the dilutions may need to be adjusted accordingly.
Our Abpromise guarantee covers the use of ab9801 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Indirect ELISA||1/1000 - 1/10000.|
|Sandwich ELISA||Use at an assay dependent dilution. Can be paired for Sandwich ELISA with Mouse monoclonal [GIF-10] to Interferon gamma (ab9805). PubMed: 9458493Used as the detection antibody in conjuction with recommended pair. sELISA can be used to determine IFN-g concentration in the cultures of human peripheral blood mononuclear cells.|
|Inhibition Assay||Use at an assay dependent dilution. Can be used to neutralize the antiviral activity of IFN-g: The bioassay was performed using the L41 cell line and mouse encephalomyocarditis virus (EMCV). Briefly, interferon was pre-incubated with the ab for 2 h at RT and then added to the cell monolayer. 24 h later cells were infected with EMCV and cultured for further 24 h. Antiviral activity of interferon was evaluated using crystal-violet staining. PAbs to respective interferons and MAb isotype control were used as controls.|
|WB||1/500 - 1/1000. Detects a band of approximately 16-21 kDa.|
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