Anti-Interferon gamma抗体[EP1109Y] (ab52746)
Key features and details
- Rabbit monoclonal [EP1109Y] to Interferon gamma
- Suitable for: WB
- Reacts with: Human, Recombinant fragment
- Isotype: IgG
Related conjugates and formulations
概述
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产品名称
Anti-Interferon gamma抗体[EP1109Y]
参阅全部 Interferon gamma 一抗 -
描述
兔单克隆抗体[EP1109Y] to Interferon gamma -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: ICC/IF or IHC-P -
种属反应性
与反应: Human, Recombinant fragment -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Human Interferon gamma recombinant protein.
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常规说明
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.15% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP1109Y -
同种型
IgG -
研究领域
相关产品
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab52746于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000 - 1/2000. Detects a band of approximately 17 kDa (predicted molecular weight: 19 kDa).
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说明 |
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WB
1/1000 - 1/2000. Detects a band of approximately 17 kDa (predicted molecular weight: 19 kDa). |
靶标
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功能
Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. -
组织特异性
Released primarily from activated T lymphocytes. -
疾病相关
In Caucasians, genetic variation in IFNG is associated with the risk of aplastic anemia (AA) [MIM:609135]. AA is a rare disease in which the reduction of the circulating blood cells results from damage to the stem cell pool in bone marrow. In most patients, the stem cell lesion is caused by an autoimmune attack. T-lymphocytes, activated by an endogenous or exogenous, and most often unknown antigenic stimulus, secrete cytokines, including IFN-gamma, which would in turn be able to suppress hematopoiesis. -
序列相似性
Belongs to the type II (or gamma) interferon family. -
翻译后修饰
Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 3458 Human
- Omim: 147570 Human
- SwissProt: P01579 Human
- Unigene: 856 Human
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别名
- IF 1 antibody
- IFG antibody
- IFI antibody
see all
图片
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Anti-Interferon gamma antibody [EP1109Y] (ab52746) at 1/1000 dilution + Human Interferon gamma recombinant protein at 0.015 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 19 kDaBlocking buffer: 5% NFDM/TBST
Diluting buffer: 5% NFDM/TBST
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Anti-Interferon gamma antibody [EP1109Y] (ab52746) at 1/2000 dilution + recombinant protein at 0.01 µg
Secondary
Goat anti-rabbit HRP labeled at 1/2000 dilution
Predicted band size: 19 kDa
Observed band size: 17 kDa why is the actual band size different from the predicted?
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab52746 被引用在 1 文献中.
- Lian X et al. Vibrio parahaemolyticus elevates interferon alpha production in intestinal-like epithelial Caco-2 cells. Can J Microbiol 53:1084-90 (2007). PubMed: 18026229