Anti-Interferon gamma抗体[D9D10] (ab11412)

Abreviews (1)Q&A (3)

概述

  • 产品名称
    Anti-Interferon gamma抗体[D9D10]
    参阅全部 Interferon gamma 一抗
  • 描述
    小鼠单克隆抗体[D9D10] to Interferon gamma
  • 宿主
    Mouse
  • 经测试应用
    适用于: Blocking, Flow Cyt, ELISA, WBmore details
  • 种属反应性
    与反应: Human, Baboon
  • 免疫原

    Full length native protein (purified) (Human).

  • 常规说明
    This product should be stored undiluted. Storage in frost free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.

性能

应用

Our Abpromise guarantee covers the use of ab11412 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Blocking Use a concentration of 1 µg/ml.
Flow Cyt 1/10 - 1/100.

Use 10 µl of the suggested working dilution to label 1 x 106 cells in 100 µl.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ELISA Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Use under non reducing condition. Predicted molecular weight: 19.9 kDa.

Use under non reducing conditions.

靶标

  • 功能
    Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.
  • 组织特异性
    Released primarily from activated T lymphocytes.
  • 疾病相关
    In Caucasians, genetic variation in IFNG is associated with the risk of aplastic anemia (AA) [MIM:609135]. AA is a rare disease in which the reduction of the circulating blood cells results from damage to the stem cell pool in bone marrow. In most patients, the stem cell lesion is caused by an autoimmune attack. T-lymphocytes, activated by an endogenous or exogenous, and most often unknown antigenic stimulus, secrete cytokines, including IFN-gamma, which would in turn be able to suppress hematopoiesis.
  • 序列相似性
    Belongs to the type II (or gamma) interferon family.
  • 翻译后修饰
    Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161.
  • 细胞定位
    Secreted.
  • Information by UniProt
  • 数据库链接
    • 别名
      • IFG antibody
      • IFI antibody
      • IFN gamma antibody
      • IFN, immune antibody
      • IFN-gamma antibody
      • IFNG antibody
      • IFNG_HUMAN antibody
      • Immune interferon antibody
      • Interferon gamma antibody
      see all

    文献

    ab11412 has not yet been referenced specifically in any publications.

    发表研究结果有使用 ab11412？请让我们知道，以便我们可以引用本数据表中的参考文章。

    客户评价及客户问答

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    4 reviews or Q&A

    10
    Votes: Highest First

    Blocking abreview for Anti-Interferon gamma antibody [D9D10]

     Excellent
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Blocking
    Sample
    Human Cultured Cells (Block of STAT1 phosphorilation in IM9 myeloma cell)
    Specification
    Block of STAT1 phosphorilation in IM9 myeloma cell
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    Username

    Dr. Roberto Bellucci

    Verified customer

    提交于 Jul 18 2012

    Thanks for your email.  Here is the 166 amino acid sequence I have for guinea pig interferon-gamma: mkytssilal qfciilsfss yycqsrftne irilknyfna dnsdvgdngt lfvgilkncq eeserkifqs qivsfyfklf ekhftdnqtv qnsmntikeq iitkffkdns snkvqafknl iqisvndehv qrqaiielk...

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    Thank you for your reply.  After reviewing our catalog, it seems that we don't have any antibodies that would be likely to cross-react with the guinea pig Interferon gamma sequence you provided.  Of the antibodies we have, the closest match I could fin...

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    Does the IFN-gamma antibody under code: ab11412 react with guinea pig?  If not, do you have any IFN-gamma antibodies that will react?

    Thank you for contacting us.  I was unable to locate an amino acid sequence for guinea pig IFN-gamma.  If you could please provide one, I would be happy to check our antibodies to see if we have one that would be likely to react.

    It states on the datasheet that this antibody recognizes only the dimeric form of the protein. Is it then necesary to run a native gel for the Western with this antibody?

    Thank you for your enquiry. The originator of the antibody confirmed that gels for Western blotting with this antibody should be run using non-reducing conditions, as we suspected. I have added this information to the datasheet for future reference. ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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