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Our Abpromise guarantee covers the use of ab9918 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use a concentration of 0.5 - 2 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to Interferon gamma (Biotin) (ab83136). This antigen affinity purified antibody, in conjunction with recommended pair as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIFN-gamma. Can be used as capture antibody when paired with ab83136.|
|WB||Use a concentration of 0.1 - 0.2 µg/ml.|
|ELISA||Use a concentration of 0.5 µg/ml. Allows the detection of 0.2 - 0.4 ng/well of recombinant mIFN-g.|
|Neutralising||Use a concentration of 0.004 - 0.006 µg/ml. for one-half maximal inhibition [ND50] of the biological activity of mIFN-g (50 pg/ml).|