重组Anti-Interferon beta抗体[EPR23341-7] (ab275880)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23341-7] to Interferon beta
- Suitable for: IP, WB
- Reacts with: Human
Related conjugates and formulations
概述
-
产品名称
Anti-Interferon beta抗体[EPR23341-7]
参阅全部 Interferon beta 一抗 -
描述
兔单克隆抗体[EPR23341-7] to Interferon beta -
宿主
Rabbit -
经测试应用
适用于: IP, WBmore details
不适用于: Flow Cyt,ICC/IF,IHC-Fr or IHC-P -
种属反应性
与反应: Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
阳性对照
- WB: Recombinant Human IFN-ß protein; A549 (transfected with 400 ng/ml poly(I:C) for 24 hours, 300ng/ml BFA was added to cells 4 hours after the start of the transfection) whole cell lysate. IP: A549 (transfected with 400 ng/ml poly(I:C) for 24 hours, 300ng/ml BFA was added to cells 4 hours after the start of the transfection) whole cell lysate.
-
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
-
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR23341-7 -
同种型
IgG -
研究领域
相关产品
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab275880于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IP |
1/30.
|
|
WB |
1/1000. Detects a band of approximately 18, 19, 22 kDa (predicted molecular weight: 22 kDa).
|
说明 |
---|
IP
1/30. |
WB
1/1000. Detects a band of approximately 18, 19, 22 kDa (predicted molecular weight: 22 kDa). |
靶标
-
功能
Has antiviral, antibacterial and anticancer activities. -
序列相似性
Belongs to the alpha/beta interferon family. -
细胞定位
Secreted. - Information by UniProt
-
数据库链接
- Entrez Gene: 3456 Human
- Omim: 147640 Human
- SwissProt: P01574 Human
- Unigene: 93177 Human
-
别名
- beta-interferon antibody
- Fibroblast interferon antibody
- IFB antibody
see all
图片
-
All lanes : Anti-Interferon beta antibody [EPR23341-7] (ab275880) at 1/1000 dilution
Lane 1 : A549 (human lung carcinoma epithelial cell) transfected with 400ng/ml poly(I:C) for 24 hours, 300ng/ml BFA was added to cells 4 hours after the start of the transfection, whole cell lysate
Lane 2 : A549 transfected with water instead of poly I:C for 24 hours, 300ng/ml BFA was added to cells 4 hours after the start of the transfection, whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 22 kDa
Observed band size: 19,22 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The molecular weight observed is consistent with what has been described in the literature (PMID:7352261).
Exposure time: 97 seconds.
-
Anti-Interferon beta antibody [EPR23341-7] (ab275880) at 1/1000 dilution + Recombinant Human IFN-beta protein, 10 ng
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution
Predicted band size: 22 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
Exposure time: 3 seconds.
-
Interferon beta was immunoprecipitated from 0.35 mg A549 (human lung carcinoma epithelial cell) (transfected with 400ng/ml poly(I:C) for 4 hours, then 300ng/ml BFA was added for the last 20 hours) whole cell lysate with ab275880 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab275880 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: A549 (transfected with 400ng/ml poly(I:C) for 4 hours, then 300ng/ml BFA was added for the last 20 hours) whole cell lysate 10 ug
Lane 2: ab275880 IP in A549 (transfected with 400ng/ml poly(I:C) for 4 hours, then 300ng/ml BFA was added for the last 20 hours) whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab275880 in A549 (transfected with 400ng/ml poly(I:C) for 4 hours, then 300ng/ml BFA was added for the last 20 hours) whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
-
SDS download
-
Datasheet download
Certificate of Compliance
文献 (0)
ab275880 尚未被引用在任何文献中。