Anti-Interferon beta抗体[7F-D3] (ab24324)

概述

  • 产品名称Anti-Interferon beta抗体[7F-D3]
    参阅全部 Interferon beta 一抗
  • 描述
    大鼠单克隆抗体[7F-D3] to Interferon beta
  • 特异性This antibody cross react with IFN beta, but not alpha or gamma.
  • 经测试应用适用于: WB, ELISA, Flow Cyt, Neutralisingmore details
  • 种属反应性
    与反应: Mouse
    不与反应: Human
  • 免疫原

    Purified mouse interferon beta.

性能

应用

Our Abpromise guarantee covers the use of ab24324 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
Flow Cyt Use a concentration of 10 µg/ml. PubMed: 24474800ab18407-Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody.
Neutralising Use at an assay dependent concentration. At a concentration of 3.6 ng/ml, this antibody neutralizes 10 U/ml of mouse IFN-b to 1 U/ml.

靶标

  • 功能Has antiviral, antibacterial and anticancer activities.
  • 序列相似性Belongs to the alpha/beta interferon family.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • beta-interferon antibody
    • Fibroblast interferon antibody
    • IFB antibody
    • IFF antibody
    • IFN beta antibody
    • IFN-beta antibody
    • IFNB 1 antibody
    • IFNB antibody
    • IFNB_HUMAN antibody
    • IFNB1 antibody
    • Interferon beta 1 fibroblast antibody
    • Interferon beta antibody
    • Interferon beta precursor antibody
    • MGC96956 antibody
    see all

Anti-Interferon beta antibody [7F-D3] (ab24324)参考文献

This product has been referenced in:
  • Wu J  et al. Strain-specific innate immune signaling pathways determine malaria parasitemia dynamics and host mortality. Proc Natl Acad Sci U S A 111:E511-20 (2014). Flow Cyt ; Mouse . Read more (PubMed: 24474800) »
  • Tang CK  et al. The chemotherapeutic agent DMXAA as a unique IRF3-dependent type-2 vaccine adjuvant. PLoS One 8:e60038 (2013). ELISA ; Mouse . Read more (PubMed: 23555875) »

See all 7 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Recombinant protein (recombinant IFNbeta AND mouse hepatocyte lysate)
Loading amount 0.5 µg
Specification recombinant IFNbeta AND mouse hepatocyte lysate
Gel Running Conditions Reduced Denaturing (10% SDS-PAGE)
Blocking step 50:50 PBS:Odyssey Blocking Buffer, Li-Cor 927-40100 as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: 24°C
Username

Abcam user community

Verified customer

提交于 Aug 28 2012

Thank you for your reply. I am very glad to be of help with this. You understand the program perfectly. I have created a code for testing Ab24324 in WB for you below. There is a 4 month window for our 100% Abreview Testing Program within which you m...

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Thank you for your reply. This clone was first developed and referenced in 1980 by Kawade, Y. J. Interferon Res. 1, 61-70 (1980). As far as I am aware it was created using purified mouse IFNB and not recombinant protein. However I do not have access...

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Thank you for your reply.

I do understand your concerns. It may be that you would want to run a western blot with this product to ensure that it is picking up IFN from your cells, it would them be possible to run a purified protein as control...

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Thank you for contacting us.

The name of the hybridoma cell line is 7F-D3 - the same as the clone number.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information. ...

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Thank you for contacting us. I am sorry that the two vials you received did not contain the full amount of the product and I apologize for the inconvenience. I have issued a free of charge replacement vial with the order number of *****. ...

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Thank you for contacting us. We have tested this antibody in indirect ELISA and neutralization assays only. We haven’t tested this antibody in sELISA in combination with other antibodies so we will not be able to recommend the suitable ELISA pair f...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (eye tissue)
Specification eye tissue
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated
Permeabilization No
Username

Abcam user community

Verified customer

提交于 Apr 20 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"