Anti-Interferon alpha 2b抗体[4E10] (HRP) (ab5258)

概述

  • 产品名称
    Anti-Interferon alpha 2b抗体[4E10] (HRP)
    参阅全部 Interferon alpha 2b 一抗
  • 描述
    小鼠单克隆抗体[4E10] to Interferon alpha 2b (HRP)
  • 偶联物
    HRP
  • 特异性
    Recognizes both human recombinant IFN-a2b and IFN-a2a. Does not react with human recombinant IFN-a5 and IFN-g.
  • 经测试应用
    适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant full length protein (Human).

  • 阳性对照
  • 常规说明

    Recommended ELISA procedure for human IFN alpha 2b determination: 1. Use 96-well Nunc Maxisorp plates for the assay. Coat microwells with 100 µl/well of antibody 9D3 (ab9386) diluted to 10 µg/ml in coating buffer (0.05 M Na-carbonate, pH 9.5). Incubate overnight at +4°C. 2. Aspirate wells and block with 150 µl/well of blocking buffer (2% BSA in PBS). Incubate at RT for 1 h. 3. Aspirate wells and wash 3 times with PBST (0.1% Tween-20 in PBS). 4. Prepare standard and sample dilutions in PBST. Recommended IFN alpha 2b concentrations: from 100 pg/ml to 100 ng/ml. Add 100 µl of each standard and sample into appropriate wells. Incubate at RT for 2 h. 5. Aspirate and wash 5 times with PBST. 6. Dilute detection antibody 4E10-HRP (ab5258) 1:1000 in PBST. Add 100 µl of diluted detection antibody to each well. Incubate at RT for 1 h. 7. Aspirate and wash 10 times with PBST. 8. Add 100 µl of substrate solution (TMB) to each well. Incubate at RT for 10-20 min. 9. Add 50 µl of stop solution (2 N H2SO4) to each well. Read absorbance at 450 nm.

    This product was changed from ascites to tissue culture supernatant on 28/11/2017. Lot numbers higher than GR308991-1 and GR308991-2 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.

性能

应用

Our Abpromise guarantee covers the use of ab5258 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
ELISA
  • 应用说明
    Sandwich ELISA: 1/1000.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    Comments:
    This antibody can be used in two-site ELISA for the determination of human IFN-a2b.
    It recognizes a different epitope than monoclonal antibody 9D3 (ab9386).
    We recommend using this antibody (ab5258) as a detection antibody.
  • 靶标

    • 功能
      Produced by macrophages, IFN-alpha have antiviral activities.
    • 序列相似性
      Belongs to the alpha/beta interferon family.
    • 细胞定位
      Secreted.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Alpha 2a interferon antibody
      • IFN alpha 2b antibody
      • IFN-alpha-2 antibody
      • IFNA antibody
      • Ifna2 antibody
      • IFNA2_HUMAN antibody
      • INFA2 antibody
      • Interferon alpha 2 antibody
      • Interferon alpha A antibody
      • Interferon alpha-2 antibody
      • Interferon alpha-A antibody
      • LeIF A antibody
      • LeIFA antibody
      see all

    实验方案

    文献

    ab5258 has not yet been referenced specifically in any publications.

    客户评价及客户问答

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Recombinant protein (E coli)
    Loading amount
    2 µg
    Specification
    E coli
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Username

    Abcam user community

    Verified customer

    提交于 Sep 13 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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