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Recombinant full length protein (Human).
The neutralising bioassay was performed using L41 cell line and mouse encephalomyocarditis virus (EMCV). Briefly, interferon was pre-incubated with the test antibodies for 2 h at RT and then added to the cell monolayer. 24 h later the cells were infected with EMCV and cultured for further 24 h. Antiviral activity of interferon was evaluated using crystal-violet staining. Polyclonal antibodies to respective interferons and irrelevant monoclonal antibody of the same isotype were used as controls.
Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab87900 as a replacement.
Our Abpromise guarantee covers the use of ab9388 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Indirect ELISA||1/500 - 1/2000.|
ab9388 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"