Anti-Interferon alpha 2b抗体[4E10] (ab9388)

概述

  • 产品名称
    Anti-Interferon alpha 2b抗体[4E10]
    参阅全部 Interferon alpha 2b 一抗
  • 描述
    小鼠单克隆抗体[4E10] to Interferon alpha 2b
  • 特异性
    Recognizes both human recombinant IFN-a2b and IFN-a2a. Does not react with human recombinant IFN-a5 and IFN-g. Does not inhibit antiviral activity of IFN-a2b in vitro.
  • 经测试应用
    适用于: Indirect ELISA, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant full length protein (Human).

  • 阳性对照
  • 常规说明

    The neutralising bioassay was performed using L41 cell line and mouse encephalomyocarditis virus (EMCV). Briefly, interferon was pre-incubated with the test antibodies for 2 h at RT and then added to the cell monolayer. 24 h later the cells were infected with EMCV and cultured for further 24 h. Antiviral activity of interferon was evaluated using crystal-violet staining. Polyclonal antibodies to respective interferons and irrelevant monoclonal antibody of the same isotype were used as controls.

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab87900 as a replacement.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 纯度
    IgG fraction
  • 克隆
    单克隆
  • 克隆编号
    4E10
  • 骨髓瘤
    NS0/1
  • 同种型
    IgG1
  • 轻链类型
    unknown
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab9388 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Indirect ELISA 1/500 - 1/2000.
WB 1/500.

靶标

  • 功能
    Produced by macrophages, IFN-alpha have antiviral activities.
  • 序列相似性
    Belongs to the alpha/beta interferon family.
  • 细胞定位
    Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Alpha 2a interferon antibody
    • IFN alpha 2b antibody
    • IFN-alpha-2 antibody
    • IFNA antibody
    • Ifna2 antibody
    • IFNA2_HUMAN antibody
    • INFA2 antibody
    • Interferon alpha 2 antibody
    • Interferon alpha A antibody
    • Interferon alpha-2 antibody
    • Interferon alpha-A antibody
    • LeIF A antibody
    • LeIFA antibody
    see all

图片

  • Immunoblot of human interferon alpha 2b using monoclonal antibody 4E10 at a dilution of 1:500 (4 µg/ml).
    Lane 1 - interferon alpha 2b
    Lane 2 - prestained standard proteins.

实验方案

文献

ab9388 has not yet been referenced specifically in any publications.

客户评价及客户问答

Thank you for contacting us. Yes, these are the same lot numbers in all cases. The number after the - just indicates the time it was aliquotted, so if the GRXXXX is the same but the dash is different you can be assured the lot is still the same. Please...

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Thank you for contacting us.

We have used this antibody under reducing and denaturing conditions in western blot. We unfortunately haven't done any comparison studies between native vs reducing conditions. I am sorry we are unable to comment o...

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Thank you for contacting us. Concentrations of the antibodies ab9386 and ab9388 are determined spectrophotometrically by an adsorbance measurement at 280 nm. The absorbance value (A280) is divided by the extiction coefficient of mouse IgG - 1.35. I hop...

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Thank you for contacting Abcam. For both ab9386 and ab9388, we currently sell them in 100ul aliquots, at a concentration of 2mg/ml. From what I can see, there are no plans to change this concentration for future lots of the antibody and since it is a m...

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Thank you for your enquiry. We currently do not have an antibody to human interferon alpha that is biotinylated. We have the following human interferon alpha antibodies: ab9660, ab9386, ab8317, ab9388, and ab5258 (HRP conjugated). If you have any more ...

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To use monoclonal antibodies 9D3 and 4E10 in sandwich ELISA for the detection of huIFN-alpha-2b, 4E10 antibody should be labeled either with horse-radish peroxidase or biotin. Monoclonal antibody 9D3 should be used as a capture antibody (for plate coat...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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