Anti-Integrin beta 8抗体(ab80673)

概述

  • 产品名称Anti-Integrin beta 8抗体
    参阅全部 Integrin beta 8 一抗
  • 描述
    兔多克隆抗体to Integrin beta 8
  • 经测试应用适用于: ELISA, IHC-P, WBmore details
  • 种属反应性
    与反应: Rat, Human
    预测可用于: Mouse, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig
  • 免疫原

    Synthetic peptide: CECTDPRSIG RFCEHCPTCY TACKENWNCM QCLHPHNLSQ AILDQCKTSC , corresponding to C terminal amino acids 611-660 of Human Integrin beta 8 (accession number: NP_002205)

  • 阳性对照
    • Human placenta membrane lysate This antibody gave a positive result in IHC in the following FFPE tissue: Human normal placenta.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • 存储溶液Constituents: 2% Sucrose, 98% PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab80673 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent concentration.

Only Peptide ELISA tested

IHC-P Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 81 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

Anti-Integrin beta 8 antibody 图像

  • IHC image of Integrin beta 8 staining in human placenta formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab80673, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Anti-Integrin beta 8 antibody (ab80673) at 1 µg/ml + Placenta lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Observed band size : 81 kDa (why is the actual band size different from the predicted?)

Anti-Integrin beta 8 antibody (ab80673)参考文献

ab80673 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Glioma)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris/EDTA buffer, pH 9
Permeabilization No
Specification Glioma
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Jun 11 2015

Application Western blot
Loading amount 400000 cells
Gel Running Conditions Reduced Denaturing (10%)
Sample Rat Cell lysate - whole cell (primary astrocytes)
Specification primary astrocytes
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Dec 22 2014

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 22°C
Sample Rat Cell (primary brain astrocytes)
Specification primary brain astrocytes
Permeabilization Yes - 0,1% Triton
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Dec 19 2014

Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing (10% gel)
Sample Human Cell lysate - whole cell (Glioma)
Specification Glioma
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Dec 18 2013

Unfortunately this antibody has not yet been tested in Flow Cytometry, so we don't have any data stating whether or not this antibody would be suitable for this application.

I am very pleased to hear you would like to accept our offer and test ab80673 in WB in mouse. This code will give you 1 freeprimary antibody before the expiration date. To redeem this offer, please submit an Abreview for WB in mouse and include this co...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"