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Synthetic peptide: CECTDPRSIG RFCEHCPTCY TACKENWNCM QCLHPHNLSQ AILDQCKTSC , corresponding to C terminal amino acids 611-660 of Human Integrin beta 8 (accession number: NP_002205)
Our Abpromise guarantee covers the use of ab80673 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.
Only Peptide ELISA tested
|IHC-P||Use a concentration of 1 µg/ml.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 81 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
IHC image of Integrin beta 8 staining in human placenta formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab80673, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre