生物素Anti-Integrin beta 7抗体[FIB504] (ab95816)
Key features and details
- Biotin Rat monoclonal [FIB504] to Integrin beta 7
- Suitable for: ICC, Flow Cyt
- Reacts with: Mouse, Human
- Conjugation: Biotin
- Isotype: IgG2a
概述
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产品名称
生物素Anti-Integrin beta 7抗体[FIB504]
参阅全部 Integrin beta 7 一抗 -
描述
生物素大鼠单克隆抗体[FIB504] to Integrin beta 7 -
宿主
Rat -
偶联物
Biotin -
经测试应用
适用于: ICC, Flow Cytmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Tissue, cells or virus corresponding to Mouse Integrin beta 7. TK1 murine t-cell lymphoma
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阳性对照
- Normal Human peripheral blood cells
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.09% Sodium azide -
Concentration information loading...
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纯度
Protein G purified -
克隆
单克隆 -
克隆编号
FIB504 -
同种型
IgG2a -
轻链类型
kappa -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab95816于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC |
Use at an assay dependent concentration.
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Flow Cyt |
Use 0.125µg for 105 cells.
Range from 10^5 to 10^8 cells/test in a final volume of 100 µl. ab18445 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
说明 |
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ICC
Use at an assay dependent concentration. |
Flow Cyt
Use 0.125µg for 105 cells. Range from 10^5 to 10^8 cells/test in a final volume of 100 µl. ab18445 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
靶标
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功能
Integrin alpha-4/beta-7 (Peyer patches-specific homing receptor LPAM-1) is an adhesion molecule that mediates lymphocyte migration and homing to gut-associated lymphoid tissue (GALT). Integrin alpha-4/beta-7 interacts with the cell surface adhesion molecules MADCAM1 which is normally expressed by the vascular endothelium of the gastrointestinal tract. Interacts also with VCAM1 and fibronectin, an extracellular matrix component. It recognizes one or more domains within the alternatively spliced CS-1 region of fibronectin. Interactions involves the tripeptide L-D-T in MADCAM1, and L-D-V in fibronectin. Binds to HIV-1 gp120, thereby allowing the virus to enter GALT, which is thought to be the major trigger of AIDS disease. Interaction would involve a tripeptide L-D-I in HIV-1 gp120. Integrin alpha-E/beta-7 (HML-1) is a receptor for E-cadherin. -
组织特异性
Expressed in a variety of leukocyte lines. -
序列相似性
Belongs to the integrin beta chain family.
Contains 1 VWFA domain. -
结构域
Domain I contains three cation-binding sites: the ligand-integrin-binding site (LIMBS), the metal ion-dependent adhesion site (MIDAS), and the adjacent to MIDAS site (ADMIDAS). In the absence of a ligand or in calcium-dependent binding, only ADMIDAS is occupied. In magnesium-dependent binding all three sites bind metal ions. LIMBS positively modify ligand binding whereas ADMIDAS negatively modify ligand binding. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 3695 Human
- Entrez Gene: 16421 Mouse
- Omim: 147559 Human
- SwissProt: P26010 Human
- SwissProt: P26011 Mouse
- Unigene: 654470 Human
- Unigene: 58 Mouse
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别名
- Gut homing receptor beta subunit antibody
- Integrin beta 7 subunit antibody
- Integrin beta-7 antibody
see all
图片
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ICC/IF image of Integrin beta 7 staining on RAW264.7 cell line using ab95816 (1:300). The cells were fixed using formaldehyde and permeabilized using 0.2% Triton X-100. The cells were blocked using 10% goat serum for 1 hour at 20°C. The cells were incubated with ab95816 (1:300) for 1 hour at 20°C. The staining was detected using Streptavedin conjugated to Alexo Fluor 647 (1:350)
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Staining of normal Human peripheral blood cells with 0.06 µg of ab95816 followed by Streptavidin PE. Cells in the lymphocyte gate were used for analysis.
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Staining of normal Human peripheral blood cells with Anti-Human CD3 APC and 0.125 µg of Rat IgG2a ? Isotype Control Biotin. Cells in the lymphocyte gate were used for analysis.
实验方案
数据表及文件
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Datasheet download
文献 (3)
ab95816 被引用在 3 文献中.
- Chai Z et al. Recombinant Viral Capsid Protein L2 (rVL2) of HPV 16 Suppresses Cell Proliferation and Glucose Metabolism via ITGB7/C/EBPß Signaling Pathway in Cervical Cancer Cell Lines. Onco Targets Ther 12:10415-10425 (2019). PubMed: 31819523
- Meerschaert J et al. Engagement of alpha4beta7 integrins by monoclonal antibodies or ligands enhances survival of human eosinophils in vitro. J Immunol 163:6217-27 (1999). PubMed: 10570314
- Tidswell M et al. Structure-function analysis of the integrin beta 7 subunit: identification of domains involved in adhesion to MAdCAM-1. J Immunol 159:1497-505 (1997). PubMed: 9233649