Anti-Integrin beta 7抗体[FIB504] (Biotin) (ab95816)

概述

  • 产品名称Anti-Integrin beta 7抗体[FIB504] (Biotin)
    参阅全部 Integrin beta 7 一抗
  • 描述
    大鼠单克隆抗体[FIB504] to Integrin beta 7 (Biotin)
  • 偶联物Biotin
  • 经测试应用适用于: ICC/IF, Flow Cytmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    TK1 murine t-cell lymphoma

  • 阳性对照
    • Normal Human peripheral blood cells

性能

应用

Our Abpromise guarantee covers the use of ab95816 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/300.
Flow Cyt Use 0.125µg for 105 cells. Range from 105 to 108 cells/test in a final volume of 100 µl.



ab18445-Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

靶标

  • 功能Integrin alpha-4/beta-7 (Peyer patches-specific homing receptor LPAM-1) is an adhesion molecule that mediates lymphocyte migration and homing to gut-associated lymphoid tissue (GALT). Integrin alpha-4/beta-7 interacts with the cell surface adhesion molecules MADCAM1 which is normally expressed by the vascular endothelium of the gastrointestinal tract. Interacts also with VCAM1 and fibronectin, an extracellular matrix component. It recognizes one or more domains within the alternatively spliced CS-1 region of fibronectin. Interactions involves the tripeptide L-D-T in MADCAM1, and L-D-V in fibronectin. Binds to HIV-1 gp120, thereby allowing the virus to enter GALT, which is thought to be the major trigger of AIDS disease. Interaction would involve a tripeptide L-D-I in HIV-1 gp120. Integrin alpha-E/beta-7 (HML-1) is a receptor for E-cadherin.
  • 组织特异性Expressed in a variety of leukocyte lines.
  • 序列相似性Belongs to the integrin beta chain family.
    Contains 1 VWFA domain.
  • 结构域Domain I contains three cation-binding sites: the ligand-integrin-binding site (LIMBS), the metal ion-dependent adhesion site (MIDAS), and the adjacent to MIDAS site (ADMIDAS). In the absence of a ligand or in calcium-dependent binding, only ADMIDAS is occupied. In magnesium-dependent binding all three sites bind metal ions. LIMBS positively modify ligand binding whereas ADMIDAS negatively modify ligand binding.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Gut homing receptor beta subunit antibody
    • Integrin beta 7 subunit antibody
    • Integrin beta-7 antibody
    • Integrin beta-P antibody
    • Integrin, beta 7 antibody
    • ITB7 antibody
    • ITB7_HUMAN antibody
    • Itgb7 antibody
    • Ly69 antibody
    • M290 IEL antigen antibody
    see all

Anti-Integrin beta 7 antibody [FIB504] (Biotin) 图像

  • ICC/IF image of Integrin beta 7 staining on RAW264.7 cell line using ab95816 (1:300). The cells were fixed using formaldehyde and permeabilized using 0.2% Triton X-100. The cells were blocked using 10% goat serum for 1 hour at 20°C. The cells were incubated with ab95816 (1:300) for 1 hour at 20°C. The staining was detected using Streptavedin conjugated to Alexo Fluor 647 (1:350)

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  • Staining of normal Human peripheral blood cells with 0.06 µg of ab95816 followed by Streptavidin PE. Cells in the lymphocyte gate were used for analysis.
  • Staining of normal Human peripheral blood cells with Anti-Human CD3 APC and 0.125 µg of Rat IgG2a ? Isotype Control Biotin. Cells in the lymphocyte gate were used for analysis.

Anti-Integrin beta 7 antibody [FIB504] (Biotin) (ab95816)参考文献

This product has been referenced in:
  • Meerschaert J  et al. Engagement of alpha4beta7 integrins by monoclonal antibodies or ligands enhances survival of human eosinophils in vitro. J Immunol 163:6217-27 (1999). Read more (PubMed: 10570314) »
  • Tidswell M  et al. Structure-function analysis of the integrin beta 7 subunit: identification of domains involved in adhesion to MAdCAM-1. J Immunol 159:1497-505 (1997). Read more (PubMed: 9233649) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Sample Mouse Cell (raw 264.7 (macrophage))
Specification raw 264.7 (macrophage)
Permeabilization Yes - 0.2% Triton X-100
Fixative Formaldehyde
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提交于 Jul 01 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"