The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/2000. Detects a band of approximately 110 kDa (predicted molecular weight: 88 kDa). Can be blocked with Integrin beta 5 peptide (786-799) (ab45697).
ELISA: This antibody gave a positive result in ELISA against the immunizing peptide. Antibody detection limit dilution 1:16,000.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Integrin alpha-V/beta-5 is a receptor for fibronectin. It recognizes the sequence R-G-D in its ligand.
Belongs to the integrin beta chain family. Contains 1 PSI domain. Contains 1 VWFA domain.