Anti-Integrin beta 5抗体(ab31327)

概述

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline. pH 7.3
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

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应用

Our Abpromise guarantee covers the use of ab31327 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
ELISA
  • 应用说明WB: 1/2000. Detects a band of approximately 110 kDa (predicted molecular weight: 88 kDa). Can be blocked with Integrin beta 5 peptide (786-799) (ab45697).
    ELISA: This antibody gave a positive result in ELISA against the immunizing peptide. Antibody detection limit dilution 1:16,000.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    Anti-Integrin beta 5 antibody 图像

    • Anti-Integrin beta 5 antibody (ab31327) at 0.1 µg/ml + Tissue lysates prepared from Human Ovary at 35 µg

      Predicted band size : 88 kDa

    Anti-Integrin beta 5 antibody (ab31327)参考文献

    ab31327 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for your enquiry.

    The integrin beta 5protein is known to be glycosylated. Some other integrin beta 5 antibodies do show a ˜100 kDa and this is probably the glycosylated form.

    The following Uniprot protein database entry ...

    Read More
    Application Western blot
    Sample Mouse Tissue lysate - whole (eyecup)
    Loading amount 20 µg
    Specification eyecup
    Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 10%
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    提交于 Jul 21 2006

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"