Anti-Integrin beta 5抗体(ab31327)

概述

  • 产品名称
    Anti-Integrin beta 5抗体
    参阅全部 Integrin beta 5 一抗
  • 描述
    山羊多克隆抗体to Integrin beta 5
  • 经测试应用
    适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Cow, Dog, Pig
  • 免疫原

    Synthetic peptide:

    (C)TFNKFNKSYNGTVD

    , corresponding to C terminal amino acids 786-799 of Human Integrin beta 5

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline. pH 7.3
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab31327 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
ELISA
  • 应用说明
    WB: 1/2000. Detects a band of approximately 110 kDa (predicted molecular weight: 88 kDa). Can be blocked with Integrin beta 5 peptide (786-799) (ab45697).
    ELISA: This antibody gave a positive result in ELISA against the immunizing peptide. Antibody detection limit dilution 1:16,000.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    图片

    • Anti-Integrin beta 5 antibody (ab31327) at 0.1 µg/ml + Tissue lysates prepared from Human Ovary at 35 µg

      Predicted band size : 88 kDa

    实验方案

    文献

    ab31327 has not yet been referenced specifically in any publications.

    客户评价及客户问答

    Thank you for your enquiry.

    The integrin beta 5protein is known to be glycosylated. Some other integrin beta 5 antibodies do show a ˜100 kDa and this is probably the glycosylated form.

    The following Uniprot protein database entry ...

    Read More
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (eyecup)
    Loading amount
    20 µg
    Specification
    eyecup
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 10%
    Username

    Abcam user community

    Verified customer

    提交于 Jul 21 2006

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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