Anti-Integrin beta 5抗体(ab15459)


  • 产品名称
    Anti-Integrin beta 5抗体
    参阅全部 Integrin beta 5 一抗
  • 描述
    兔多克隆抗体to Integrin beta 5
  • 宿主
  • 经测试应用
    适用于: ICC/IF, IHC-Fr, IP, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Hamster
  • 免疫原

    Synthetic peptide. This information is considered to be commercially sensitive.

  • 阳性对照
    • Small intestine, bone marrow and colon carcinoma HeLa Cells Rat gastric epithelial


  • 形式
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • 存储溶液
    pH: 7.6
    Preservative: 0.1% Sodium azide
    Constituents: 0.0268% PBS, 1% BSA
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
  • 同种型
  • 研究领域


Our Abpromise guarantee covers the use of ab15459 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/20.
IHC-Fr Use at an assay dependent concentration. PubMed: 22025551
IP Use at an assay dependent concentration. PubMed: 22025551
IHC-P 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.



  • ICC/IF image of ab15459 stained HeLa cells (ab150035ab15459), 1µg/ml) overnight at +4°C. The secondary antibody (green) was a goat anti-rabbit Alexa Fluor® 488 (IgG; H+L) ab150077) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Human small intestine (ab29276) stained with anti-Integrin beta 5 antibody, ab15459 by Immunohistochemistry (FFPE-sections).

  • ab15459 staining Integrin beta 5 in Rat gastric epithelial cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton and blocked with 0.25% serum free protein for 20 minutes at 28°C. Samples were incubated with primary antibody (1/50 in antibody diluent) for 2 hours at 28°C. An FITC-conjugated Goat anti-IgG polyclonal (1/80) was used as the secondary antibody. Nuclei were counterstained with propidium iodide (ab14083).

    See Abreview


This product has been referenced in:
  • Lin Z  et al. Integrin-ß5, a miR-185-targeted gene, promotes hepatocellular carcinoma tumorigenesis by regulating ß-catenin stability. J Exp Clin Cancer Res 37:17 (2018). Read more (PubMed: 29386044) »
  • Zhou X  et al. An integrin antagonist (MK-0429) decreases proteinuria and renal fibrosis in the ZSF1 rat diabetic nephropathy model. Pharmacol Res Perspect 5:N/A (2017). Read more (PubMed: 28971604) »

See all 19 Publications for this product


Western blot
Human Cell lysate - whole cell (Glioma)
Gel Running Conditions
Reduced Denaturing (10% gel)
Loading amount
25 µg
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

提交于 May 12 2015

Unfortunately, this antibody is not against the extracellular domain and so will not be suitable for your application.

We have not tested any of our antibodies in vivo and therefore cannot guarantee that any of these will work on live unpermea...

Read More

Vielen Dank für Ihre Anfrage.

Ich habe Ihnen unser Subcellular Fractionation Protokoll an diese Email gehängt, mit dem Sie eine Isolierung der Zellmembran durchführen können.

Desweiteren habe ich Ihnen unseren &...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Immunocytochemistry/ Immunofluorescence
Rat Cell (gastric epithelial cell)
gastric epithelial cell
Yes - 0.1% Triton
Blocking step
DAKO serum free protein blocker as blocking agent for 20 minute(s) · Concentration: 0.25% · Temperature: 28°C

Dr. J Chai

Verified customer

提交于 Apr 14 2012

Thank you for your inquiry.

Yes, the blocking buffer that was used to generate that image was a combination of 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilize the cells and block non-specific protei...

Read More

Thank you for contacting us. The blocking step requires you to choose either 1%BSA or 10% normal serum for blocking. Incubation should be either for one hour at room temperature or overnight at 4 degrees.

I hope this information is helpfu...

Read More

Thank you for bringing this to our attention. Can you please send the order numbers for each antibody that has discrepancy between the delivered datasheet and the online datasheet? Please also send a brief description of the issues you are having, if a...

Read More

Thank you for contacting us. We would guarantee this antibody to work in both human and mouse tissue sections.  If you had any difficulty in IHC-P with these two species, we would be able to replace or refund the products for you within 6 months of you...

Read More

Thank you for contacting us. The antibody ab15459 has not yet been tested in flow cytometry. If you like to try that, we have a testing discount program you can take advantage of. For UNTESTED species and/or applications, we have estab...

Read More

Thank you for contacting us. The C-terminal region is located in the cytoplasm. Depending on your studies, a permeabilization step would be necessary. I hope this information is helpful to you. Please do not hesitate to contact us if you need any m...

Read More

1-10 of 12 Abreviews or Q&A