Anti-Integrin beta 4 (phospho Y1494)抗体(ab29043)

概述

  • 产品名称Anti-Integrin beta 4 (phospho Y1494)抗体
    参阅全部 Integrin beta 4 一抗
  • 描述
    兔多克隆抗体to Integrin beta 4 (phospho Y1494)
  • 特异性ab29043 recognises all three isoforms of Integrin beta 4 phosphorilated at tyrosine 1494 (beta 4A, beta 4B and beta 4C).
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic phospho peptide (coupled to KLH) corresponding to amino acid residues around tyrosine 1494 of human integrin beta 4.

  • 阳性对照
    • A431 cells treated with pervanadate.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.05% Sodium Azide
    Constituents: 50% Glycerol, PBS, 1mg/ml BSA
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明ab29043 was cross adsorbed to phospho tyrosine coupled to agarose prior to immunogen affinity purification (without carrier protein).
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab29043 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
ELISA
  • 应用说明ELISA: 1/4000.
    WB: 1/2000 Block the membrane in 5% BSA (fraction V). Predicted molecular weight: 202 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Integrin alpha-6/beta-4 is a receptor for laminin. It plays a critical structural role in the hemidesmosome of epithelial cells.
    • 组织特异性Integrin alpha-6/beta-4 is predominantly expressed by epithelia. Isoform beta-4D is also expressed in colon and placenta. Isoform beta-4E is also expressed in epidermis, lung, duodenum, heart, spleen and stomach.
    • 疾病相关Defects in ITGB4 are a cause of epidermolysis bullosa letalis with pyloric atresia (EB-PA) [MIM:226730]; also known as junctional epidermolysis bullosa with pyloric atresia (PA-JEB) or aplasia cutis congenita with gastrointestinal atresia. EB-PA is an autosomal recessive, frequently lethal, epidermolysis bullosa with variable involvement of skin, nails, mucosa, and with variable effects on the digestive system. It is characterized by mucocutaneous fragility, aplasia cutis congenita, and gastrointestinal atresia, which most commonly affects the pylorus. Pyloric atresia is a primary manifestation rather than a scarring process secondary to epidermolysis bullosa.
      Defects in ITGB4 are a cause of generalized atrophic benign epidermolysis bullosa (GABEB) [MIM:226650]. GABEB is a non-lethal, adult form of junctional epidermolysis bullosa characterized by life-long blistering of the skin, associated with hair and tooth abnormalities.
    • 序列相似性Belongs to the integrin beta chain family.
      Contains 1 Calx-beta domain.
      Contains 4 fibronectin type-III domains.
      Contains 1 PSI domain.
      Contains 1 VWFA domain.
    • 结构域The fibronectin type-III-like domains bind BPAG1 and plectin and probably also recruit BP230.
    • 细胞定位Membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • CD 104 antibody
      • CD104 antibody
      • CD104 antigen antibody
      • gp150 antibody
      • Integrin beta 4 subunit antibody
      • Integrin beta-4 antibody
      • ITB4_HUMAN antibody
      • ITG B4 antibody
      • ITGB 4 antibody
      • Itgb4 antibody
      see all

    Anti-Integrin beta 4 (phospho Y1494) antibody 图像

    • All lanes : Anti-Integrin beta 4 (phospho Y1494) antibody (ab29043)

      Lane 1 : A431 (25 µg/lane) serum starved overnight
      Lane 2 : A431 (25 µg/lane) serum treated with pervanadate (1 mM) for 30 min with absence of peptide
      Lane 3 : A431 (25 µg/lane) serum treated with pervanadate (1 mM) for 30 min with presence of phospho-integrin-beta4 (Tyr-1494) peptide
      Lane 4 : A431 (25 µg/lane) serum treated with pervanadate (1 mM) for 30 min with presence of phospho-integrin-beta4 (Tyr-1526) peptide


      Predicted band size : 202 kDa

    Anti-Integrin beta 4 (phospho Y1494) antibody (ab29043)参考文献

    ab29043 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab29043.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"