Binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit to form the 80S initiation complex in the cytoplasm. May behave as a stimulatory translation initiation factor downstream insulin/growth factors. Is also involved in ribosome biogenesis. Associates with pre-60S subunits in the nucleus and is involved in its nuclear export. Cytoplasmic release of TIF6 from 60S subunits and nuclear relocalization is promoted by a RACK1 (GNB2L1)-dependent protein kinase C activity.
Expressed at very high levels in colon carcinoma with lower levels in normal colon and ileum and lowest levels in kidney and muscle (at protein level).
Belongs to the eIF-6 family.
Phosphorylation at Ser-174 and Ser-175 promotes nuclear export.
Cytoplasm. Nucleus > nucleolus. Shuttles between cytoplasm and nucleus/nucleolus.
Western blot - integrin beta 4 binding protein antibody (ab109593)
All lanes : Anti-Integrin beta 4 binding protein antibody (ab109593) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Lane 4 : NIH3T3 whole cell lysate at 50 µg
Developed using the ECL technique
Predicted band size : 27 kDa
Exposure time : 3 minutes
Immunoprecipitation - integrin beta 4 binding protein antibody (ab109593)
Detection of integrin beta 4 binding protein by Western Blot of Immunprecipitate.
Lane 1: ab109593 at 1µg/ml staining integrin beta 4 binding protein in HeLa whole cell lysate immunoprecipitated using ab109593 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Lane 2: Control IgG
Detection: Chemiluminescence with exposure time of 10 seconds.