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recombinant protein containing amino acid residues in the cytoplasmic region of human integrin beta 4.
Our Abpromise guarantee covers the use of ab29042 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 202 kDa. In 5% non fat milk, PBS, 0.04% Tween 20 for 1 hour at room temperature.|
|Flow Cyt||1/100. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.|
|ICC/IF||Use at an assay dependent concentration. PubMed: 21042878|
Immunofluorescence analysis of rat 804G cells, staining Integrin beta 4 with ab29042.
Cells were fixed in 4% formaldehyde in PBS for 8 minutes, washed thoroughly in PBS, and permeabilized in 0.1% Triton X-100 in PBS for 10 minutes. Samples were incubated with primary antibody at 37°C for 1 hour. The cells on coverslips were washed with PBS, and AlexaFluor®597-conjugated secondary antibodies were applied for 1 hour at 37°C.
Overlay histogram showing A431 cells stained with ab29042 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum (ab7481) / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab29042, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was a goat anti-mouse DyLight® 488 (IgG; H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.