概述

  • 产品名称Anti-Integrin beta 4抗体[ASC-8]
    参阅全部 Integrin beta 4 一抗
  • 描述
    小鼠单克隆抗体[ASC-8] to Integrin beta 4
  • 经测试应用适用于: IP, IHC-Fr, Flow Cyt, Inhibition Assaymore details
    不适用于: IHC-P
  • 种属反应性
    与反应: Human
  • 免疫原

    Human squamous cell carcinoma cells (SCC9)

  • 阳性对照
    • IHC-Fr: Human epithelial tissues (including tongue, foreskin and ovary). Flow Cyt: Human SCC9, SKOV-3, HUVEC cell lines. Does not stain monocytes, neutrophils, lymphocytes, T cells, B cells or platelets.

性能

  • 形式Liquid
  • 存放说明Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.1% Sodium Azide
    Constituents: 0.25M Sodium chloride, 0.02M PBS, pH 7.6
  • Concentration information loading...
  • 纯度Protein A purified
  • 克隆单克隆
  • 克隆编号ASC-8
  • 同种型IgG1
  • 轻链类型kappa
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab77801 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use at an assay dependent dilution. Precipitates PAGE bands of 66, 76, 120, 135 and 200kDa (reduced) or 66, 110, 125, 130 and 190kDa (non-reduced) from surface-biotinylated SCC9 cells.
IHC-Fr Use at an assay dependent dilution.
Flow Cyt Use 1µg for 106 cells.
Inhibition Assay Use at an assay dependent dilution. Inhibits SCC9 cell adhesion to human and EHS laminin and laminin GD-2 peptide, but not to type IV collagen or fibronectin. Does not inhibit SKOV-3 cell adhesion to laminin, fibronectin or type IV collagen.
  • 应用说明Is unsuitable for IHC-P.
  • 靶标

    • 功能Integrin alpha-6/beta-4 is a receptor for laminin. It plays a critical structural role in the hemidesmosome of epithelial cells.
    • 组织特异性Integrin alpha-6/beta-4 is predominantly expressed by epithelia. Isoform beta-4D is also expressed in colon and placenta. Isoform beta-4E is also expressed in epidermis, lung, duodenum, heart, spleen and stomach.
    • 疾病相关Defects in ITGB4 are a cause of epidermolysis bullosa letalis with pyloric atresia (EB-PA) [MIM:226730]; also known as junctional epidermolysis bullosa with pyloric atresia (PA-JEB) or aplasia cutis congenita with gastrointestinal atresia. EB-PA is an autosomal recessive, frequently lethal, epidermolysis bullosa with variable involvement of skin, nails, mucosa, and with variable effects on the digestive system. It is characterized by mucocutaneous fragility, aplasia cutis congenita, and gastrointestinal atresia, which most commonly affects the pylorus. Pyloric atresia is a primary manifestation rather than a scarring process secondary to epidermolysis bullosa.
      Defects in ITGB4 are a cause of generalized atrophic benign epidermolysis bullosa (GABEB) [MIM:226650]. GABEB is a non-lethal, adult form of junctional epidermolysis bullosa characterized by life-long blistering of the skin, associated with hair and tooth abnormalities.
    • 序列相似性Belongs to the integrin beta chain family.
      Contains 1 Calx-beta domain.
      Contains 4 fibronectin type-III domains.
      Contains 1 PSI domain.
      Contains 1 VWFA domain.
    • 结构域The fibronectin type-III-like domains bind BPAG1 and plectin and probably also recruit BP230.
    • 细胞定位Membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • CD 104 antibody
      • CD104 antibody
      • CD104 antigen antibody
      • gp150 antibody
      • Integrin beta 4 subunit antibody
      • Integrin beta-4 antibody
      • ITB4_HUMAN antibody
      • ITG B4 antibody
      • ITGB 4 antibody
      • Itgb4 antibody
      see all

    Anti-Integrin beta 4 antibody [ASC-8] 图像

    • Overlay histogram showing A431 cells stained with ab77801 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab77801, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    Anti-Integrin beta 4 antibody [ASC-8] (ab77801)参考文献

    This product has been referenced in:
    • Chen Q  et al. Down-regulation of Gli transcription factor leads to the inhibition of migration and invasion of ovarian cancer cells via integrin ß4-mediated FAK signaling. PLoS One 9:e88386 (2014). WB ; Human . Read more (PubMed: 24533083) »

    See 1 Publication for this product

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