Anti-Integrin beta 4抗体[450-9D] (FITC) (ab22486)

概述

  • 产品名称
    Anti-Integrin beta 4抗体[450-9D] (FITC)
    参阅全部 Integrin beta 4 一抗
  • 描述
    小鼠单克隆抗体[450-9D] to Integrin beta 4 (FITC)
  • 偶联物
    FITC. Ex: 493nm, Em: 528nm
  • 特异性
    ab22486 recognises the integrin beta 4 expressed on epithelial cells, Schwann cells and various tumour cell lines.
  • 经测试应用
    适用于: IHC-Fr, Flow Cytmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length native protein (purified) (Human): Purified a6b4 integrin from A431 cells

  • 表位
    the epitope is in the extracellular region of Integrin beta 4
  • 阳性对照
    • Human tonsil

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 0.1% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 纯度
    Protein G purified
  • 纯化说明
    ab22486 has been purified by affinity chromatography on Protein G from tissue culture supernatant.
  • 克隆
    单克隆
  • 克隆编号
    450-9D
  • 骨髓瘤
    Sp2/0
  • 同种型
    IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab22486 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr Use at an assay dependent concentration.
Flow Cyt 1/10. Use 10ul of the suggested working dilution to label 106 cells in 100ul.



ab91356-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

靶标

  • 功能
    Integrin alpha-6/beta-4 is a receptor for laminin. It plays a critical structural role in the hemidesmosome of epithelial cells.
  • 组织特异性
    Integrin alpha-6/beta-4 is predominantly expressed by epithelia. Isoform beta-4D is also expressed in colon and placenta. Isoform beta-4E is also expressed in epidermis, lung, duodenum, heart, spleen and stomach.
  • 疾病相关
    Defects in ITGB4 are a cause of epidermolysis bullosa letalis with pyloric atresia (EB-PA) [MIM:226730]; also known as junctional epidermolysis bullosa with pyloric atresia (PA-JEB) or aplasia cutis congenita with gastrointestinal atresia. EB-PA is an autosomal recessive, frequently lethal, epidermolysis bullosa with variable involvement of skin, nails, mucosa, and with variable effects on the digestive system. It is characterized by mucocutaneous fragility, aplasia cutis congenita, and gastrointestinal atresia, which most commonly affects the pylorus. Pyloric atresia is a primary manifestation rather than a scarring process secondary to epidermolysis bullosa.
    Defects in ITGB4 are a cause of generalized atrophic benign epidermolysis bullosa (GABEB) [MIM:226650]. GABEB is a non-lethal, adult form of junctional epidermolysis bullosa characterized by life-long blistering of the skin, associated with hair and tooth abnormalities.
  • 序列相似性
    Belongs to the integrin beta chain family.
    Contains 1 Calx-beta domain.
    Contains 4 fibronectin type-III domains.
    Contains 1 PSI domain.
    Contains 1 VWFA domain.
  • 结构域
    The fibronectin type-III-like domains bind BPAG1 and plectin and probably also recruit BP230.
  • 细胞定位
    Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CD 104 antibody
    • CD104 antibody
    • CD104 antigen antibody
    • gp150 antibody
    • Integrin beta 4 subunit antibody
    • Integrin beta-4 antibody
    • ITB4_HUMAN antibody
    • ITG B4 antibody
    • ITGB 4 antibody
    • Itgb4 antibody
    see all

图片

  • Immunohistochemistry (Frozen sections) analysis of human tonsil tissue section labeling Integrin beta 4 with ab22486. 

文献

This product has been referenced in:
  • James JL  et al. Isolation and characterisation of a novel trophoblast side-population from first trimester placentae. Reproduction 150:449-62 (2015). Read more (PubMed: 26248480) »

See 1 Publication for this product

客户评价及客户问答

Further to previous correspondence I have been in touch with the source of this antibody. I am concerned that this antibody has in fact NOT been tested and shown to work by western blotting. It is FITC labeled and therefore my concern was that the fluo...

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Thank you for your enquiry. I am sorry to hear that you have been having difficulties with this antibody. I appreciate the controls that you have in place and that you are detecting Integrin beta 4 using an alternatively sourced antibody. Please ca...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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