Anti-Integrin beta 4抗体[346-11A] (ab25254)


  • 产品名称Anti-Integrin beta 4抗体[346-11A]
    参阅全部 Integrin beta 4 一抗
  • 描述
    大鼠单克隆抗体[346-11A] to Integrin beta 4
  • 特异性ab25254 recognises Integrin ß4 chain (CD104), Mr 200 kDa.
  • 经测试应用适用于: Flow Cyt, ICC/IF, IHC-Fr, IP, WBmore details
  • 种属反应性
    与反应: Mouse
  • 免疫原

    Full length native protein (purified) (Mouse) - Tumour associated antigen TSP 180 - immunoaffinity purified from a transplantable BALB/c mouse lung cell carcinoma.


  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液100mM borate buffered saline, pH 8.0.
  • Concentration information loading...
  • 纯度IgG fraction
  • 克隆单克隆
  • 克隆编号346-11A
  • 同种型IgG2a
  • 轻链类型kappa
  • 研究领域


Our Abpromise guarantee covers the use of ab25254 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use at an assay dependent concentration. ab18450-Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
ICC/IF Use at an assay dependent concentration. PubMed: 20094062
IHC-Fr Use a concentration of 1 - 5 µg/ml.
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 202 kDa.


  • 功能Integrin alpha-6/beta-4 is a receptor for laminin. It plays a critical structural role in the hemidesmosome of epithelial cells.
  • 组织特异性Integrin alpha-6/beta-4 is predominantly expressed by epithelia. Isoform beta-4D is also expressed in colon and placenta. Isoform beta-4E is also expressed in epidermis, lung, duodenum, heart, spleen and stomach.
  • 疾病相关Defects in ITGB4 are a cause of epidermolysis bullosa letalis with pyloric atresia (EB-PA) [MIM:226730]; also known as junctional epidermolysis bullosa with pyloric atresia (PA-JEB) or aplasia cutis congenita with gastrointestinal atresia. EB-PA is an autosomal recessive, frequently lethal, epidermolysis bullosa with variable involvement of skin, nails, mucosa, and with variable effects on the digestive system. It is characterized by mucocutaneous fragility, aplasia cutis congenita, and gastrointestinal atresia, which most commonly affects the pylorus. Pyloric atresia is a primary manifestation rather than a scarring process secondary to epidermolysis bullosa.
    Defects in ITGB4 are a cause of generalized atrophic benign epidermolysis bullosa (GABEB) [MIM:226650]. GABEB is a non-lethal, adult form of junctional epidermolysis bullosa characterized by life-long blistering of the skin, associated with hair and tooth abnormalities.
  • 序列相似性Belongs to the integrin beta chain family.
    Contains 1 Calx-beta domain.
    Contains 4 fibronectin type-III domains.
    Contains 1 PSI domain.
    Contains 1 VWFA domain.
  • 结构域The fibronectin type-III-like domains bind BPAG1 and plectin and probably also recruit BP230.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CD 104 antibody
    • CD104 antibody
    • CD104 antigen antibody
    • gp150 antibody
    • Integrin beta 4 subunit antibody
    • Integrin beta-4 antibody
    • ITB4_HUMAN antibody
    • ITG B4 antibody
    • ITGB 4 antibody
    • Itgb4 antibody
    see all

Anti-Integrin beta 4 antibody [346-11A] 图像

  • This image shows a acetone fixed frozen section of BALB/c mouse intestine. The basal layer stains intensely (arrow).
  • Immunohistochemistry (Frozen sections) analysis of BALB/c mouse frozen intestine. Right: Integrin beta 4 labeled with ab25254 followed by Goat Anti-Rat IgG(H+L), Mouse ads-BIOT, and Streptavidin-FITC. Left: Isotype Control, Rat IgG2a-UNLB followed by Goat Anti-Rat IgG(H+L), Mouse ads-BIOT, and Streptavidin-FITC. Blue - DAPI

  • ab25254 staining mouse small intestine tissue sections by IHC-Fr.  Sections were PFA fixed and blocked in 3% BSA for 1 hour at 37°C prior to incubatition with the primary antibody, diluted 1/500, for 12 hours at 4°C.  A Cy3® conjugated goat anti-rat antibody, diluted 1/200, was used as the secondary.

    See Abreview

  • ab25254 staining Intergrin beta 4 in mouse colon cells by Flow Cytometry. The sample was incubated with the primary antibody (1/100) for 30 minutes at 20°C. An undiluted Alexa Fluor® 647-conjugated goat anti-rat IgG polyclonal was used as the secondary antibody. Gating Strategy: Exclude small cells/debris.

    See Abreview

Anti-Integrin beta 4 antibody [346-11A] (ab25254)参考文献

This product has been referenced in:
  • Grudzien-Nogalska E  et al. CPEB1 promotes differentiation and suppresses EMT in mammary epithelial cells. J Cell Sci 127:2326-38 (2014). Read more (PubMed: 24634508) »
  • Fatehullah A  et al. Cell and tissue polarity in the intestinal tract during tumourigenesis: cells still know the right way up, but tissue organization is lost. Philos Trans R Soc Lond B Biol Sci 368:20130014 (2013). Read more (PubMed: 24062584) »

See all 5 Publications for this product

Product Wall

Application Flow Cytometry
Permeabilization No
Sample Mouse Cell (mouse colon)
Specification mouse colon
Gating Strategy exclude small cells/debris
Preparation Cell harvesting/tissue preparation method: crypt isolation
Sample buffer: dmem

Abcam user community

Verified customer

提交于 Oct 03 2014

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 20°C
Sample Mouse Cell (colon epithelial)
Specification colon epithelial
Permeabilization No
Fixative Formaldehyde

Abcam user community

Verified customer

提交于 Aug 28 2014


ab25254 - ***
ab110167 - ***

Expiration date: ***

I am very pleased to hear you would like to accept our offer and test ab25254 in flow cytometry, and ab110167 on mouse samples. To redeem this offer...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Small intestine)
Specification Small intestine
Fixative Paraformaldehyde
Permeabilization No
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 37°C

Abcam user community

Verified customer

提交于 Apr 03 2008