Anti-Integrin beta 3 (phospho Y773)抗体(ab38460)

概述

  • 产品名称Anti-Integrin beta 3 (phospho Y773)抗体
    参阅全部 Integrin beta 3 一抗
  • 描述
    兔多克隆抗体to Integrin beta 3 (phospho Y773)
  • 特异性ab38460 recognises endogenous levels of Integrin beta 3 only when phosphorylated at tyrosine 773.
  • 经测试应用适用于: WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic phosphopeptide derived from human Integrin beta 3 around the phosphorylation site of tyrosine 773 (PLYpKE)

  • 阳性对照
    • Human breast carcinoma tissue, HL-60 cells.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab38460 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
ELISA
IHC-P
  • 应用说明ELISA: 1/10,000.
    IHC-P: 1/50 - 1/100.
    WB: 1/500 - 1/1000. Predicted molecular weight: 87 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Integrin alpha-V/beta-3 is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrin alpha-IIb/beta-3 is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. Integrins alpha-IIb/beta-3 and alpha-V/beta-3 recognize the sequence R-G-D in a wide array of ligands. Integrin alpha-IIb/beta-3 recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha-IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial surface. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
    • 组织特异性Isoform beta-3A and isoform beta-3C are widely expressed. Isoform beta-3A is specifically expressed in osteoblast cells; isoform beta-3C is specifically expressed in prostate and testis.
    • 疾病相关Defects in ITGB3 are a cause of Glanzmann thrombasthenia (GT) [MIM:273800]; also known as thrombasthenia of Glanzmann and Naegeli. GT is the most common inherited disease of platelets. It is an autosomal recessive disorder characterized by mucocutaneous bleeding of mild-to-moderate severity and the inability of this integrin to recognize macromolecular or synthetic peptide ligands. GT has been classified clinically into types I and II. In type I, platelets show absence of the glycoprotein IIb/beta-3 complexes at their surface and lack fibrinogen and clot retraction capability. In type II, the platelets express the glycoprotein IIb/beta-3 complex at reduced levels (5-20% controls), have detectable amounts of fibrinogen, and have low or moderate clot retraction capability. The platelets of GT 'variants' have normal or near normal (60-100%) expression of dysfunctional receptors.
    • 序列相似性Belongs to the integrin beta chain family.
      Contains 1 VWFA domain.
    • 翻译后修饰Phosphorylated on tyrosine residues in response to thrombin-induced platelet aggregation. Probably involved in outside-in signaling. A peptide (AA 740-762) is capable of binding GRB2 only when both Tyr-773 and Tyr-785 are phosphorylated. Phosphorylation of Thr-779 inhibits SHC binding.
    • 细胞定位Membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • BDPLT16 antibody
      • BDPLT2 antibody
      • CD 61 antibody
      • CD61 antibody
      • CD61 antigen antibody
      • GP3A antibody
      • GPIIIa antibody
      • GT antibody
      • HPA 1 antibody
      • HPA 4 antibody
      • Integrin beta 3 (platelet glycoprotein IIIa antigen CD61) antibody
      • Integrin beta chain beta 3 antibody
      • Integrin beta-3 antibody
      • ITB3_HUMAN antibody
      • ITG B3 antibody
      • ITGB 3 antibody
      • ITGB3 antibody
      • NAIT antibody
      • Platelet fibrinogen receptor beta subunit antibody
      • Platelet fibrinogen receptor, beta subunit antibody
      • Platelet glycoprotein IIIa antibody
      • Platelet glycoprotein IIIa precursor antibody
      • Platelet membrane glycoprotein IIIa antibody
      • PTP antibody
      see all

    Anti-Integrin beta 3 (phospho Y773) antibody 图像

    • All lanes : Anti-Integrin beta 3 (phospho Y773) antibody (ab38460) at 1/500 dilution

      Lane 1 : Untreated extracts from HL-60 cells
      Lane 2 : Hydrogen peroxide treated extracts from HL-60 cells

      Lysates/proteins at 30 µg per lane.


      Predicted band size : 87 kDa
      Observed band size : >118 kDa (why is the actual band size different from the predicted?)
    • Immunohistochemical analysis of Integrin beta 3 (phospho Y773) expression in paraffin embedded human breast carcinoma tissue sections using 1/50 ab38460. Left: untreated sample. Right: Sample pretreated with phosphopeptide (negative control).

    Anti-Integrin beta 3 (phospho Y773) antibody (ab38460)参考文献

    This product has been referenced in:
    • Mattheij NJ  et al. Dual mechanism of integrin aIIbß3 closure in procoagulant platelets. J Biol Chem 288:13325-36 (2013). Read more (PubMed: 23519467) »
    • Sun Y  et al. Carbon disulfide exposure at peri-implantation disrupts embryo implantation by decreasing integrin ß3 expression in the uterine tissue of pregnant mice. Chem Biol Interact 206:126-133 (2013). Read more (PubMed: 24025711) »

    See all 3 Publications for this product

    Product Wall

    Thank you for contacting us. Ab38460 was tested on HL-60 cells treated with 100 uM H2O2 for 30 min. I hope this helps, please let me know if you need any additional information or assistance.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"