Anti-Integrin beta 3抗体[MHF4] (ab20146)


  • 产品名称Anti-Integrin beta 3抗体[MHF4]
    参阅全部 Integrin beta 3 一抗
  • 描述
    小鼠单克隆抗体[MHF4] to Integrin beta 3
  • 特异性MHF4 may be used to identify osteoclasts in tissue sections and bone cell suspensions.
  • 经测试应用适用于: IP, Flow Cyt, IHC-Frmore details
  • 种属反应性
    与反应: Rat, Human
  • 免疫原

    Cell preparation: A bone cell suspension containing osteoclasts, osteoblasts and bone marrow cells obtained from the long bones of newborn Sprague Dawley rats.

  • 常规说明A heterodimeric molecule with a characteristic shift in apparent molecular mass on reduction (137/78 kDa nonreduced; 118/100 kDa reduced).



Our Abpromise guarantee covers the use of ab20146 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt
  • 应用说明Flow Cyt: Use at an assay dependent dilution.
    IHC-Fr: Use at an assay dependent dilution.
    Note: samples should be acetone fixed.

    IP: Use at an assay dependent dilution.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Integrin alpha-V/beta-3 is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrin alpha-IIb/beta-3 is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. Integrins alpha-IIb/beta-3 and alpha-V/beta-3 recognize the sequence R-G-D in a wide array of ligands. Integrin alpha-IIb/beta-3 recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha-IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial surface. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
    • 组织特异性Isoform beta-3A and isoform beta-3C are widely expressed. Isoform beta-3A is specifically expressed in osteoblast cells; isoform beta-3C is specifically expressed in prostate and testis.
    • 疾病相关Defects in ITGB3 are a cause of Glanzmann thrombasthenia (GT) [MIM:273800]; also known as thrombasthenia of Glanzmann and Naegeli. GT is the most common inherited disease of platelets. It is an autosomal recessive disorder characterized by mucocutaneous bleeding of mild-to-moderate severity and the inability of this integrin to recognize macromolecular or synthetic peptide ligands. GT has been classified clinically into types I and II. In type I, platelets show absence of the glycoprotein IIb/beta-3 complexes at their surface and lack fibrinogen and clot retraction capability. In type II, the platelets express the glycoprotein IIb/beta-3 complex at reduced levels (5-20% controls), have detectable amounts of fibrinogen, and have low or moderate clot retraction capability. The platelets of GT 'variants' have normal or near normal (60-100%) expression of dysfunctional receptors.
    • 序列相似性Belongs to the integrin beta chain family.
      Contains 1 VWFA domain.
    • 翻译后修饰Phosphorylated on tyrosine residues in response to thrombin-induced platelet aggregation. Probably involved in outside-in signaling. A peptide (AA 740-762) is capable of binding GRB2 only when both Tyr-773 and Tyr-785 are phosphorylated. Phosphorylation of Thr-779 inhibits SHC binding.
    • 细胞定位Membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • BDPLT16 antibody
      • BDPLT2 antibody
      • CD 61 antibody
      • CD61 antibody
      • CD61 antigen antibody
      • GP3A antibody
      • GPIIIa antibody
      • GT antibody
      • HPA 1 antibody
      • HPA 4 antibody
      • Integrin beta 3 (platelet glycoprotein IIIa antigen CD61) antibody
      • Integrin beta chain beta 3 antibody
      • Integrin beta-3 antibody
      • ITB3_HUMAN antibody
      • ITG B3 antibody
      • ITGB 3 antibody
      • ITGB3 antibody
      • NAIT antibody
      • Platelet fibrinogen receptor beta subunit antibody
      • Platelet fibrinogen receptor, beta subunit antibody
      • Platelet glycoprotein IIIa antibody
      • Platelet glycoprotein IIIa precursor antibody
      • Platelet membrane glycoprotein IIIa antibody
      • PTP antibody
      see all

    Anti-Integrin beta 3 antibody [MHF4] (ab20146)参考文献

    This product has been referenced in:
    • Ponceau A  et al. aII-spectrin regulates invadosome stability and extracellular matrix degradation. PLoS One 10:e0120781 (2015). Read more (PubMed: 25830635) »
    • Helfrich MH  et al. Integrins on rat osteoclasts: characterization of two monoclonal antibodies (F4 and F11) to rat beta 3. J Bone Miner Res 7:345-51 (1992). Read more (PubMed: 1585837) »

    See all 2 Publications for this product

    Product Wall

    Toutes nos garanties d'applications et espèces pour chaque produit sont spécifiées dans la fiche technique, et sont mises à jour régulièrement. Comme indiqué sur la fiche technique (, l'anti-Integrin beta 3 référence ab201...

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