Anti-Integrin beta 3抗体[EPR2342] (ab119992)


  • 产品名称Anti-Integrin beta 3抗体[EPR2342]
    参阅全部 Integrin beta 3 一抗
  • 描述
    兔单克隆抗体[EPR2342] to Integrin beta 3
  • 经测试应用适用于: WB, IPmore details
    不适用于: Flow Cyt,ICC or IHC-P
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Integrin beta 3 aa 750-850.

  • 阳性对照
    • THP-1 treated with TPA, HUVEC, Human spleen and Human plasma lysates
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A trial size is available to purchase for this antibody.



Our Abpromise guarantee covers the use of ab119992 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Detects a band of approximately 110 kDa (predicted molecular weight: 87 kDa).
IP 1/50.
  • 应用说明Is unsuitable for Flow Cyt,ICC or IHC-P.
  • 靶标

    • 功能Integrin alpha-V/beta-3 is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrin alpha-IIb/beta-3 is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. Integrins alpha-IIb/beta-3 and alpha-V/beta-3 recognize the sequence R-G-D in a wide array of ligands. Integrin alpha-IIb/beta-3 recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha-IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial surface. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
    • 组织特异性Isoform beta-3A and isoform beta-3C are widely expressed. Isoform beta-3A is specifically expressed in osteoblast cells; isoform beta-3C is specifically expressed in prostate and testis.
    • 疾病相关Defects in ITGB3 are a cause of Glanzmann thrombasthenia (GT) [MIM:273800]; also known as thrombasthenia of Glanzmann and Naegeli. GT is the most common inherited disease of platelets. It is an autosomal recessive disorder characterized by mucocutaneous bleeding of mild-to-moderate severity and the inability of this integrin to recognize macromolecular or synthetic peptide ligands. GT has been classified clinically into types I and II. In type I, platelets show absence of the glycoprotein IIb/beta-3 complexes at their surface and lack fibrinogen and clot retraction capability. In type II, the platelets express the glycoprotein IIb/beta-3 complex at reduced levels (5-20% controls), have detectable amounts of fibrinogen, and have low or moderate clot retraction capability. The platelets of GT 'variants' have normal or near normal (60-100%) expression of dysfunctional receptors.
    • 序列相似性Belongs to the integrin beta chain family.
      Contains 1 VWFA domain.
    • 翻译后修饰Phosphorylated on tyrosine residues in response to thrombin-induced platelet aggregation. Probably involved in outside-in signaling. A peptide (AA 740-762) is capable of binding GRB2 only when both Tyr-773 and Tyr-785 are phosphorylated. Phosphorylation of Thr-779 inhibits SHC binding.
    • 细胞定位Membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • BDPLT16 antibody
      • BDPLT2 antibody
      • CD 61 antibody
      • CD61 antibody
      • CD61 antigen antibody
      • GP3A antibody
      • GPIIIa antibody
      • GT antibody
      • HPA 1 antibody
      • HPA 4 antibody
      • Integrin beta 3 (platelet glycoprotein IIIa antigen CD61) antibody
      • Integrin beta chain beta 3 antibody
      • Integrin beta-3 antibody
      • ITB3_HUMAN antibody
      • ITG B3 antibody
      • ITGB 3 antibody
      • ITGB3 antibody
      • NAIT antibody
      • Platelet fibrinogen receptor beta subunit antibody
      • Platelet fibrinogen receptor, beta subunit antibody
      • Platelet glycoprotein IIIa antibody
      • Platelet glycoprotein IIIa precursor antibody
      • Platelet membrane glycoprotein IIIa antibody
      • PTP antibody
      see all

    Anti-Integrin beta 3 antibody [EPR2342] 图像

    • All lanes : Anti-Integrin beta 3 antibody [EPR2342] (ab119992) at 1/1000 dilution

      Lane 1 : TPA treated THP1 cell lysate
      Lane 2 : HUVEC cell lysate
      Lane 3 : Human spleen lysate
      Lane 4 : Human plasma lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size : 87 kDa
      Observed band size : 110 kDa (why is the actual band size different from the predicted?)

    Anti-Integrin beta 3 antibody [EPR2342] (ab119992)参考文献

    This product has been referenced in:
    • Jiang M  et al. Changes in tension regulates proliferation and migration of fibroblasts by remodeling expression of ECM proteins. Exp Ther Med 12:1542-1550 (2016). WB ; Human . Read more (PubMed: 27588075) »
    • Klausen C  et al. Cell motility and spreading are suppressed by HOXA4 in ovarian cancer cells: possible involvement of beta1 integrin. Mol Cancer Res 7:1425-37 (2009). Read more (PubMed: 19723874) »

    See all 2 Publications for this product

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    Thank you for contacting us.

    After checking our antibodies, ab131056 may be suitable for your purposes. The immunogen is 100% homologous with mouse and only 3 amino acids are not conserved in the 3b and 3c isoforms so it should show cross-rea...

    Read More

    Based on sequence analysis, the ab119992 is expected to detect only the isoform 3A of Integrin beta 3.
    I hope this is helpful. Please let me know if you have any further questions.