RabMAb

Anti-Integrin beta 3抗体[EPR2342] (ab119992)

概述

  • 产品名称Anti-Integrin beta 3抗体[EPR2342]
    参阅全部 Integrin beta 3 一抗
  • 描述
    兔单克隆抗体[EPR2342] to Integrin beta 3
  • 经测试应用适用于: WB, IPmore details
    不适用于: Flow Cyt,ICC or IHC-P
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Integrin beta 3 aa 750-850.

  • 阳性对照
    • THP-1 treated with TPA, HUVEC, Human spleen and Human plasma lysates
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A trial size is available to purchase for this antibody.

性能

应用

Our Abpromise guarantee covers the use of ab119992 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Detects a band of approximately 110 kDa (predicted molecular weight: 87 kDa).
IP 1/50.
  • 应用说明Is unsuitable for Flow Cyt,ICC or IHC-P.
  • 靶标

    • 功能Integrin alpha-V/beta-3 is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrin alpha-IIb/beta-3 is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. Integrins alpha-IIb/beta-3 and alpha-V/beta-3 recognize the sequence R-G-D in a wide array of ligands. Integrin alpha-IIb/beta-3 recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha-IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial surface. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
    • 组织特异性Isoform beta-3A and isoform beta-3C are widely expressed. Isoform beta-3A is specifically expressed in osteoblast cells; isoform beta-3C is specifically expressed in prostate and testis.
    • 疾病相关Defects in ITGB3 are a cause of Glanzmann thrombasthenia (GT) [MIM:273800]; also known as thrombasthenia of Glanzmann and Naegeli. GT is the most common inherited disease of platelets. It is an autosomal recessive disorder characterized by mucocutaneous bleeding of mild-to-moderate severity and the inability of this integrin to recognize macromolecular or synthetic peptide ligands. GT has been classified clinically into types I and II. In type I, platelets show absence of the glycoprotein IIb/beta-3 complexes at their surface and lack fibrinogen and clot retraction capability. In type II, the platelets express the glycoprotein IIb/beta-3 complex at reduced levels (5-20% controls), have detectable amounts of fibrinogen, and have low or moderate clot retraction capability. The platelets of GT 'variants' have normal or near normal (60-100%) expression of dysfunctional receptors.
    • 序列相似性Belongs to the integrin beta chain family.
      Contains 1 VWFA domain.
    • 翻译后修饰Phosphorylated on tyrosine residues in response to thrombin-induced platelet aggregation. Probably involved in outside-in signaling. A peptide (AA 740-762) is capable of binding GRB2 only when both Tyr-773 and Tyr-785 are phosphorylated. Phosphorylation of Thr-779 inhibits SHC binding.
    • 细胞定位Membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • BDPLT16 antibody
      • BDPLT2 antibody
      • CD 61 antibody
      • CD61 antibody
      • CD61 antigen antibody
      • GP3A antibody
      • GPIIIa antibody
      • GT antibody
      • HPA 1 antibody
      • HPA 4 antibody
      • Integrin beta 3 (platelet glycoprotein IIIa antigen CD61) antibody
      • Integrin beta chain beta 3 antibody
      • Integrin beta-3 antibody
      • ITB3_HUMAN antibody
      • ITG B3 antibody
      • ITGB 3 antibody
      • ITGB3 antibody
      • NAIT antibody
      • Platelet fibrinogen receptor beta subunit antibody
      • Platelet fibrinogen receptor, beta subunit antibody
      • Platelet glycoprotein IIIa antibody
      • Platelet glycoprotein IIIa precursor antibody
      • Platelet membrane glycoprotein IIIa antibody
      • PTP antibody
      see all

    Anti-Integrin beta 3 antibody [EPR2342] 图像

    • All lanes : Anti-Integrin beta 3 antibody [EPR2342] (ab119992) at 1/1000 dilution

      Lane 1 : TPA treated THP1 cell lysate
      Lane 2 : HUVEC cell lysate
      Lane 3 : Human spleen lysate
      Lane 4 : Human plasma lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 87 kDa
      Observed band size : 110 kDa (why is the actual band size different from the predicted?)

    Anti-Integrin beta 3 antibody [EPR2342] (ab119992)参考文献

    This product has been referenced in:
    • Jiang M  et al. Changes in tension regulates proliferation and migration of fibroblasts by remodeling expression of ECM proteins. Exp Ther Med 12:1542-1550 (2016). WB ; Human . Read more (PubMed: 27588075) »
    • Inaba J  et al. Lunasin sensitivity in non-small cell lung cancer cells is linked to suppression of integrin signaling and changes in histone acetylation. Int J Mol Sci 15:23705-24 (2014). WB ; Human . Read more (PubMed: 25530619) »

    See all 3 Publications for this product

    Product Wall

    Thank you for contacting us.

    After checking our antibodies, ab131056 may be suitable for your purposes. The immunogen is 100% homologous with mouse and only 3 amino acids are not conserved in the 3b and 3c isoforms so it should show cross-rea...

    Read More

    Based on sequence analysis, the ab119992 is expected to detect only the isoform 3A of Integrin beta 3.
    I hope this is helpful. Please let me know if you have any further questions.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"