Anti-Integrin beta 1抗体[JB1B] (ab30388)


  • 产品名称Anti-Integrin beta 1抗体[JB1B]
    参阅全部 Integrin beta 1 一抗
  • 描述
    小鼠单克隆抗体[JB1B] to Integrin beta 1
  • 特异性We have data to indicate that this antibody may not cross react with Rat. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • 经测试应用适用于: Celluar Activation, IHC-P, Flow Cyt, IHC-Fr, ELISA, IP, WBmore details
  • 种属反应性
    与反应: Human, Pig, Cynomolgus Monkey, Rhesus monkey
  • 免疫原

    Purified Human beta 1 integrin.



Our Abpromise guarantee covers the use of ab30388 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Celluar Activation Use at an assay dependent concentration. PubMed: 7513261
IHC-P 1/100.
Flow Cyt Use 1µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
IHC-Fr Use a concentration of 5 µg/ml.
ELISA Use a concentration of 0.1 - 1 µg/ml.
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 88 kDa.


  • 功能Integrins alpha-1/beta-1, alpha-2/beta-1, alpha-10/beta-1 and alpha-11/beta-1 are receptors for collagen. Integrins alpha-1/beta-1 and alpha-2/beta-2 recognize the proline-hydroxylated sequence G-F-P-G-E-R in collagen. Integrins alpha-2/beta-1, alpha-3/beta-1, alpha-4/beta-1, alpha-5/beta-1, alpha-8/beta-1, alpha-10/beta-1, alpha-11/beta-1 and alpha-V/beta-1 are receptors for fibronectin. Alpha-4/beta-1 recognizes one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. Integrin alpha-5/beta-1 is a receptor for fibrinogen. Integrin alpha-1/beta-1, alpha-2/beta-1, alpha-6/beta-1 and alpha-7/beta-1 are receptors for lamimin. Integrin alpha-4/beta-1 is a receptor for VCAM1. It recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-9/beta-1 is a receptor for VCAM1, cytotactin and osteopontin. It recognizes the sequence A-E-I-D-G-I-E-L in cytotactin. Integrin alpha-3/beta-1 is a receptor for epiligrin, thrombospondin and CSPG4. Alpha-3/beta-1 may mediate with LGALS3 the stimulation by CSPG4 of endothelial cells migration. Integrin alpha-V/beta-1 is a receptor for vitronectin. Beta-1 integrins recognize the sequence R-G-D in a wide array of ligands. Isoform 2 interferes with isoform 1 resulting in a dominant negative effect on cell adhesion and migration (in vitro). When associated with alpha-7/beta-1 integrin, regulates cell adhesion and laminin matrix deposition. Involved in promoting endothelial cell motility and angiogenesis. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process and the formation of mineralized bone nodules. May be involved in up-regulation of the activity of kinases such as PKC via binding to KRT1. Together with KRT1 and RACK1, serves as a platform for SRC activation or inactivation. Plays a mechanistic adhesive role during telophase, required for the successful completion of cytokinesis. Integrin alpha-3/beta-1 provides a docking site for FAP (seprase) at invadopodia plasma membranes in a collagen-dependent manner and hence may participate in the adhesion, formation of invadopodia and matrix degradation processes, promoting cell invasion. ITGA4:ITGB1 binds to fractalkine (CX3CL1) and may act as its coreceptor in CX3CR1-dependent fractalkine signaling (PubMed:23125415, PubMed:24789099). ITGA4:ITGB1 and ITGA5:ITGB1 bind to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1 (PubMed:18635536, PubMed:25398877).
    Isoform 5: Isoform 5 displaces isoform 1 in striated muscles.
    (Microbial infection) Integrin ITGA2:ITGB1 acts as a receptor for human echoviruses 1 and 8 (PubMed:8411387). Acts as a receptor for cytomegalovirus/HHV-5 (PubMed:20660204). Acts as a receptor for Epstein-Barr virus/HHV-4 (PubMed:17945327). Integrin ITGA5:ITGB1 acts as a receptor for human parvovirus B19 (PubMed:12907437). Integrin ITGA2:ITGB1 acts as a receptor for human rotavirus (PubMed:12941907). Acts as a receptor for mammalian reovirus (PubMed:16501085). In case of HIV-1 infection, integrin ITGA5:ITGB1 binding to extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions (PubMed:10397733).
  • 组织特异性Isoform 1 is widely expressed, other isoforms are generally coexpressed with a more restricted distribution. Isoform 2 is expressed in skin, liver, skeletal muscle, cardiac muscle, placenta, umbilical vein endothelial cells, neuroblastoma cells, lymphoma cells, hepatoma cells and astrocytoma cells. Isoform 3 and isoform 4 are expressed in muscle, kidney, liver, placenta, cervical epithelium, umbilical vein endothelial cells, fibroblast cells, embryonal kidney cells, platelets and several blood cell lines. Isoform 4, rather than isoform 3, is selectively expressed in peripheral T-cells. Isoform 3 is expressed in non-proliferating and differentiated prostate gland epithelial cells and in platelets, on the surface of erythroleukemia cells and in various hematopoietic cell lines. Isoform 5 is expressed specifically in striated muscle (skeletal and cardiac muscle).
  • 序列相似性Belongs to the integrin beta chain family.
    Contains 1 VWFA domain.
  • 翻译后修饰The cysteine residues are involved in intrachain disulfide bonds.
  • 细胞定位Cell membrane, sarcolemma. Cell junction. In cardiac muscle, isoform 5 is found in costameres and intercalated disks and Cell membrane. Cell projection, invadopodium membrane. Cell projection, ruffle membrane. Recycling endosome. Melanosome. Cleavage furrow. Cell projection, lamellipodium. Cell projection, ruffle. Isoform 2 does not localize to focal adhesions. Highly enriched in stage I melanosomes. Located on plasma membrane of neuroblastoma NMB7 cells. In a lung cancer cell line, in prometaphase and metaphase, localizes diffusely at the membrane and in a few intracellular vesicles. In early telophase, detected mainly on the matrix-facing side of the cells. By mid-telophase, concentrated to the ingressing cleavage furrow, mainly to the basal side of the furrow. In late telophase, concentrated to the extending protrusions formed at the opposite ends of the spreading daughter cells, in vesicles at the base of the lamellipodia formed by the separating daughter cells. Colocalizes with ITGB1BP1 and metastatic suppressor protein NME2 at the edge or peripheral ruffles and lamellipodia during the early stages of cell spreading on fibronectin or collagen. Translocates from peripheral focal adhesions sites to fibrillar adhesions in a ITGB1BP1-dependent manner. Enriched preferentially at invadopodia, cell membrane protrusions that correspond to sites of cell invasion, in a collagen-dependent manner. Localized at plasma and ruffle membranes in a collagen-independent manner.
  • Information by UniProt
  • 数据库链接
  • 别名
    • beta1 integrin antibody
    • CD29 antibody
    • Fibronectin receptor subunit beta antibody
    • FNRB antibody
    • Glycoprotein IIa antibody
    • GP IIa antibody
    • GPIIA antibody
    • Integrin beta-1 antibody
    • integrin VLA-4 beta subunit antibody
    • Integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12) antibody
    • ITB1_HUMAN antibody
    • ITGB1 antibody
    • MDF2 antibody
    • MSK12 antibody
    • OTTHUMP00000019420 antibody
    • Very late activation protein, beta polypeptide antibody
    • VLA BETA antibody
    • VLA-4 subunit beta antibody
    • VLA-BETA antibody
    • VLAB antibody
    • VLAbeta antibody
    see all

Anti-Integrin beta 1 antibody [JB1B] 图像

  • ab30388 staining Integrin beta 1 in human skin tissue by Immunohistochemistry (Frozen sections).
    Tissue was fixed with acetone, blocked using 0.25% casein for 10 minutes at 20°C, then incubated with ab30388 at a 1/50 dilution for 1 hour at 20°C. The secondary used was an Alexa-Fluor 488 conjugated goat anti-mouse IgG2a, used at a 1/200 dilution.

    See Abreview

  • ab30388 staining Integrin beta 1 in Pig skin tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded tissue sections). The sections were fixed in formaldehyde and subjected to Heat-mediated antigen retrieval in 10 mM citrate pH 6.0 prior to permeabilization with 0.05% Triton X-100/PBS blocking with 5% fetal bovine serum in PBS for 1 hour at 25°C. The primary antibody was diluted 1/100 in the blocking solution and incubated with the sample for 16 hours at 4°C. A biotin-conjugated Goat anti-Mouse IgG was used as the secondary antibody, diluted 1/10000.

Anti-Integrin beta 1 antibody [JB1B] (ab30388)参考文献

This product has been referenced in:
  • Sandri C  et al. The R-Ras/RIN2/Rab5 complex controls endothelial cell adhesion and morphogenesis via active integrin endocytosis and Rac signaling. Cell Res 22:1479-501 (2012). ELISA ; Human . Read more (PubMed: 22825554) »
  • Trulsson M  et al. HAMLET Binding to a-Actinin Facilitates Tumor Cell Detachment. PLoS One 6:e17179 (2011). ICC/IF ; Human . Read more (PubMed: 21408150) »

See all 4 Publications for this product

Product Wall

Please forward the following publication to customer, there are many markers listed and we have antibodies against these. Customer might need to make decision based on their requirements.

Read More

Thank you for contacting us.
We would recommend following antibodies for IP of integrin beta 1.
ab24693, can be used for IP of both human and mouse protein.
ab7168, ab30388, ab77803, and ab78502 can be used for IPing human beta 1 integri...

Read More

Gracias por contactarnos.

Varios marcadores pueden usarse para la detección de condrocitos mediante immunohistoquímica. Solo algunos de ellos se han testado en especie canina, el resto me temo que no se han testado, por lo que s...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Pig Tissue sections (skin tissue)
Specification skin tissue
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10 mM citrate, pH 6.0
Permeabilization Yes - 0.05% triton-X100/PBS
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

提交于 Nov 10 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application ELISA
Type Direct
Sample Human Cell (Human artelial endothelial cells)
Specification Human artelial endothelial cells
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Mrs. Elena Astanina

Verified customer

提交于 Jul 26 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (Skin)
Specification Skin
Fixative Acetone
Permeabilization No
Blocking step Casein as blocking agent for 10 minute(s) · Concentration: 0.25% · Temperature: 20°C

Dr. Vaughan Feisst

Verified customer

提交于 Jul 22 2011