Anti-Integrin beta 1抗体[4B7R] (ab3167)


  • 产品名称Anti-Integrin beta 1抗体[4B7R]
    参阅全部 Integrin beta 1 一抗
  • 描述
    小鼠单克隆抗体[4B7R] to Integrin beta 1
  • 经测试应用适用于: WB, Flow Cyt, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Human melanoma V+B2 cell line.

  • 阳性对照
    • Tonsil.



Our Abpromise guarantee covers the use of ab3167 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration. Detects a band of approximately 100 kDa (predicted molecular weight: 88 kDa).
Flow Cyt Use at an assay dependent concentration.

(paraformaldehyde or methanol fixed cells)

ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IHC-P Use at an assay dependent concentration. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.
ICC/IF Use at an assay dependent concentration.


  • 功能Integrins alpha-1/beta-1, alpha-2/beta-1, alpha-10/beta-1 and alpha-11/beta-1 are receptors for collagen. Integrins alpha-1/beta-1 and alpha-2/beta-2 recognize the proline-hydroxylated sequence G-F-P-G-E-R in collagen. Integrins alpha-2/beta-1, alpha-3/beta-1, alpha-4/beta-1, alpha-5/beta-1, alpha-8/beta-1, alpha-10/beta-1, alpha-11/beta-1 and alpha-V/beta-1 are receptors for fibronectin. Alpha-4/beta-1 recognizes one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. Integrin alpha-5/beta-1 is a receptor for fibrinogen. Integrin alpha-1/beta-1, alpha-2/beta-1, alpha-6/beta-1 and alpha-7/beta-1 are receptors for lamimin. Integrin alpha-4/beta-1 is a receptor for VCAM1. It recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-9/beta-1 is a receptor for VCAM1, cytotactin and osteopontin. It recognizes the sequence A-E-I-D-G-I-E-L in cytotactin. Integrin alpha-3/beta-1 is a receptor for epiligrin, thrombospondin and CSPG4. Alpha-3/beta-1 may mediate with LGALS3 the stimulation by CSPG4 of endothelial cells migration. Integrin alpha-V/beta-1 is a receptor for vitronectin. Beta-1 integrins recognize the sequence R-G-D in a wide array of ligands. Isoform 2 interferes with isoform 1 resulting in a dominant negative effect on cell adhesion and migration (in vitro). When associated with alpha-7/beta-1 integrin, regulates cell adhesion and laminin matrix deposition. Involved in promoting endothelial cell motility and angiogenesis. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process and the formation of mineralized bone nodules. May be involved in up-regulation of the activity of kinases such as PKC via binding to KRT1. Together with KRT1 and RACK1, serves as a platform for SRC activation or inactivation. Plays a mechanistic adhesive role during telophase, required for the successful completion of cytokinesis. Integrin alpha-3/beta-1 provides a docking site for FAP (seprase) at invadopodia plasma membranes in a collagen-dependent manner and hence may participate in the adhesion, formation of invadopodia and matrix degradation processes, promoting cell invasion. ITGA4:ITGB1 binds to fractalkine (CX3CL1) and may act as its coreceptor in CX3CR1-dependent fractalkine signaling (PubMed:23125415, PubMed:24789099). ITGA4:ITGB1 and ITGA5:ITGB1 bind to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1 (PubMed:18635536, PubMed:25398877).
    Isoform 5: Isoform 5 displaces isoform 1 in striated muscles.
    (Microbial infection) Integrin ITGA2:ITGB1 acts as a receptor for human echoviruses 1 and 8 (PubMed:8411387). Acts as a receptor for cytomegalovirus/HHV-5 (PubMed:20660204). Acts as a receptor for Epstein-Barr virus/HHV-4 (PubMed:17945327). Integrin ITGA5:ITGB1 acts as a receptor for human parvovirus B19 (PubMed:12907437). Integrin ITGA2:ITGB1 acts as a receptor for human rotavirus (PubMed:12941907). Acts as a receptor for mammalian reovirus (PubMed:16501085). In case of HIV-1 infection, integrin ITGA5:ITGB1 binding to extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions (PubMed:10397733).
  • 组织特异性Isoform 1 is widely expressed, other isoforms are generally coexpressed with a more restricted distribution. Isoform 2 is expressed in skin, liver, skeletal muscle, cardiac muscle, placenta, umbilical vein endothelial cells, neuroblastoma cells, lymphoma cells, hepatoma cells and astrocytoma cells. Isoform 3 and isoform 4 are expressed in muscle, kidney, liver, placenta, cervical epithelium, umbilical vein endothelial cells, fibroblast cells, embryonal kidney cells, platelets and several blood cell lines. Isoform 4, rather than isoform 3, is selectively expressed in peripheral T-cells. Isoform 3 is expressed in non-proliferating and differentiated prostate gland epithelial cells and in platelets, on the surface of erythroleukemia cells and in various hematopoietic cell lines. Isoform 5 is expressed specifically in striated muscle (skeletal and cardiac muscle).
  • 序列相似性Belongs to the integrin beta chain family.
    Contains 1 VWFA domain.
  • 翻译后修饰The cysteine residues are involved in intrachain disulfide bonds.
  • 细胞定位Cell membrane, sarcolemma. Cell junction. In cardiac muscle, isoform 5 is found in costameres and intercalated disks and Cell membrane. Cell projection, invadopodium membrane. Cell projection, ruffle membrane. Recycling endosome. Melanosome. Cleavage furrow. Cell projection, lamellipodium. Cell projection, ruffle. Isoform 2 does not localize to focal adhesions. Highly enriched in stage I melanosomes. Located on plasma membrane of neuroblastoma NMB7 cells. In a lung cancer cell line, in prometaphase and metaphase, localizes diffusely at the membrane and in a few intracellular vesicles. In early telophase, detected mainly on the matrix-facing side of the cells. By mid-telophase, concentrated to the ingressing cleavage furrow, mainly to the basal side of the furrow. In late telophase, concentrated to the extending protrusions formed at the opposite ends of the spreading daughter cells, in vesicles at the base of the lamellipodia formed by the separating daughter cells. Colocalizes with ITGB1BP1 and metastatic suppressor protein NME2 at the edge or peripheral ruffles and lamellipodia during the early stages of cell spreading on fibronectin or collagen. Translocates from peripheral focal adhesions sites to fibrillar adhesions in a ITGB1BP1-dependent manner. Enriched preferentially at invadopodia, cell membrane protrusions that correspond to sites of cell invasion, in a collagen-dependent manner. Localized at plasma and ruffle membranes in a collagen-independent manner.
  • Information by UniProt
  • 数据库链接
  • 别名
    • beta1 integrin antibody
    • CD29 antibody
    • Fibronectin receptor subunit beta antibody
    • FNRB antibody
    • Glycoprotein IIa antibody
    • GP IIa antibody
    • GPIIA antibody
    • Integrin beta-1 antibody
    • integrin VLA-4 beta subunit antibody
    • Integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12) antibody
    • ITB1_HUMAN antibody
    • ITGB1 antibody
    • MDF2 antibody
    • MSK12 antibody
    • OTTHUMP00000019420 antibody
    • Very late activation protein, beta polypeptide antibody
    • VLA BETA antibody
    • VLA-4 subunit beta antibody
    • VLA-BETA antibody
    • VLAB antibody
    • VLAbeta antibody
    see all

Anti-Integrin beta 1 antibody [4B7R] 图像

  • Image courtesy of the human protein atlas.

    Immunofluorescent staining of human cell line A-431 with ab3167 at 1/100 shows positivity in plasma membrane.

  • Image courtesy of the human protein atlas.

    Immunohistochemical staining of human colon with ab3167 at 1/100 shows moderate cytoplasmic positivity in glandular cells.

  • Image courtesy of Human Protein Atlas

    ab3167 staining Integrin beta 1 in Human skin, showing a distinct and strong staining pattern at the basal cells of the squamous epithelium that contrasts with the reticular layer. Paraffin embedded human skin tissue was incubated with ab3167 (1/35 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.
    ab3167 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at







  • ab14404 staining Integrin beta 1 in Human Mesenchymal Stem Cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with PFA, permeabilized with 0.1% Triton X-100 in PBS and blocked with 10% Serum for 30 minutes at 20°C. Samples were incubated with primary antibody (1/100 in PBS + 1% BSA/0.1% Triton X-100) for 2 hour at 20°C. An Alexa Fluor®594-conjugated Goat anti-mouse IgG polyclonal(1/400) was used as the secondary antibody.

    See Abreview

  • Anti-Integrin beta 1 antibody [4B7R] (ab3167) at 5 µg/ml + HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg

    Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size : 88 kDa
    Observed band size : 100 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 37 kDa,40 kDa,50 kDa. We are unsure as to the identity of these extra bands.
    It is probable that this protein runs higher than the predicted MW as it likely to be glycosylated.
  • Overlay histogram showing MCF-7 cells stained with ab3167 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3167, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was a goat anti-mouse DyLight® 488 (IgG; H+L) (ab96879) at 1/250 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF-7 cells fixed with methanol (5 min) used under the same conditions.

    Please note that Abcam do not have data for use of this antibody on non-fixed cells. We welcome any customer feedback.

Anti-Integrin beta 1 antibody [4B7R] (ab3167)参考文献

This product has been referenced in:
  • Chang W  et al. Gene expression profiling-derived immunohistochemistry signature with high prognostic value in colorectal carcinoma. Gut N/A:N/A (2013). IHC ; Human . Read more (PubMed: 24173294) »
  • Schwartz MP  et al. A quantitative comparison of human HT-1080 fibrosarcoma cells and primary human dermal fibroblasts identifies a 3D migration mechanism with properties unique to the transformed phenotype. PLoS One 8:e81689 (2013). Read more (PubMed: 24349113) »

See all 8 Publications for this product

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Thank you for your enquiry and your interest in our products.

I can confirm that ab3167 has been tested and characterized only on human samples - no data available regarding murine samples.

However, we have some other antibodies aga...

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Thank you for contacting us. As I mentioned on the phone, we have tested a number of our antibodies for storage at room temperature and even up to 37C for a week and found that it does not impact the antibody quality. Please find the testing data attac...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Human Mesenchymal Stem Cells)
Specification Human Mesenchymal Stem Cells
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% Triton X-100
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 20°C

Abcam user community

Verified customer

提交于 Jul 23 2012

Unfortunately we do not have any any other information about the portion of the protein that this antibody was raised against.

Thank you for your enquiry. Both ab35 and ab3167 have been tested and shown to work using immunohistochemistry on paraffin sections. You are correct that these antibodies do indeed seem similar. Indeed because the clone numbers; [4B7] and [4B7R] ar...

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Thank you for your phone call. I regret to inform you that concerning the antibodies that you are interested in, we do not have any information regarding the epitopes. If you have any more questions, please contact us again.