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Immunization of beta 6 knockout mice with wild type murine keratinocytes.
Our Abpromise guarantee covers the use of ab77906 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration. because this antibody is an IgG2a, protein G or rabbit anti mouse IgG is helpful for capture.|
|Flow Cyt||Use at an assay dependent concentration. Flow cytometry should be done with buffers containing 1 mM Mg, since the epitope recognized by this antibody is divalent cation dependent.
ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
|Blocking||Use a concentration of 100 µg/ml. Inhibition of alpha V beta 6 mediated cell adhesion and cell migration are achieved at a concentration of 100 µg/mL using beta 6 transfected cells tested against TGF beta LAP.|