RabMAb

Anti-Integrin alpha V抗体[EPR5583] (ab124968)

概述

  • 产品名称Anti-Integrin alpha V抗体[EPR5583]
    参阅全部 Integrin alpha V 一抗
  • 描述
    兔单克隆抗体[EPR5583] to Integrin alpha V
  • 经测试应用适用于: WB, Flow Cytmore details
    不适用于: ICC,IHC-P or IP
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Integrin alpha V aa 1000-1100 (intracellular).
    Database link: P06756

  • 阳性对照
    • A549, A431, C6, RAW264.7, PC12, and NIH 3T3 cell lysates; A549 cells.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

应用

Our Abpromise guarantee covers the use of ab124968 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Detects a band of approximately 25, 150 kDa (predicted molecular weight: 86, 116 kDa).
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • 应用说明Is unsuitable for ICC,IHC-P or IP.
  • 靶标

    • 功能The alpha-V integrins are receptors for vitronectin, cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin and vWF. They recognize the sequence R-G-D in a wide array of ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
    • 序列相似性Belongs to the integrin alpha chain family.
      Contains 7 FG-GAP repeats.
    • 细胞定位Membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • antigen identified by monoclonal antibody L230 antibody
      • CD 51 antibody
      • CD51 antibody
      • DKFZp686A08142 antibody
      • integrin alpha V beta 3 antibody
      • integrin alpha-V antibody
      • Integrin alpha-V light chain antibody
      • integrin alphaVbeta3 antibody
      • integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51) antibody
      • ITAV_HUMAN antibody
      • ITGAV antibody
      • MSK 8 antibody
      • Msk8 antibody
      • Vitronectin receptor subunit alpha antibody
      • VNRA antibody
      • VTNR antibody
      see all

    Anti-Integrin alpha V antibody [EPR5583] 图像

    • All lanes : Anti-Integrin alpha V antibody [EPR5583] (ab124968) at 1/1000 dilution

      Lane 1 : A549 cell lysates
      Lane 2 : A431 cell lysates
      Lane 3 : C6 cell lysates
      Lane 4 : RAW264.7 cell lysates
      Lane 5 : PC12 cell lysates
      Lane 6 : NIH 3T3 cell lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 86, 116 kDa
    • ab124968 at 1/10 dilution staining Integrin alpha V in permeabilized A549 cells by Flow cytometry (red) or a rabbit IgG (negative) (green).

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    Anti-Integrin alpha V antibody [EPR5583] (ab124968)参考文献

    ab124968 has not yet been referenced specifically in any publications.

    Product Wall

    The higher MW band (˜ 150 KDa) on the blot for ab124968 is full length Integrin alpha-V, and the lower band (˜ 25 KDa) is Integrin alpha-V light chain (http://www.uniprot.org/uniprot/P06756). At this time we have not run any experiments to characterize...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"