Human mucosal lymphocyte antigen 1, alpha subunit antibody
Integrin alpha E heavy chain antibody
Integrin alpha E light chain antibody
Integrin alpha E, epithelial-associated antibody
Integrin alpha E1 antibody
Integrin alpha IEL antibody
Integrin alpha M290 antibody
Integrin alpha-E heavy chain antibody
Integrin alpha-IEL antibody
Integrin, alpha E (antigen CD103, human mucosal lymphocyte antigen 1; alpha polypeptide) antibody
Integrin, alpha E antibody
Mucosal lymphocyte 1 antigen antibody
Mucosal lymphocyte antigen 1 antibody
Anti-Integrin alpha E antibody 图像
Western blot - Integrin alpha E antibody (ab93848)
All lanes : Anti-Integrin alpha E antibody (ab93848) at 1/250 dilution
Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate Lane 2 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 4 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate Lane 5 : Human small intestine tissue lysate - total protein (ab29276) Lane 6 : Human colon tissue lysate - total protein (ab30051)
Lysates/proteins at 10 µg per lane.
Secondary Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution developed using the ECL technique
Immunocytochemistry/ Immunofluorescence - Integrin alpha E antibody (ab93848)
ICC/IF image of ab93848 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab93848, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) HeLa and MCF7 cells at 5µg/ml.
Anti-Integrin alpha E antibody (ab93848)参考文献
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