Anti-Integrin alpha 9+beta 1抗体[Y9A2] (ab27947)

概述

  • 产品名称
    Anti-Integrin alpha 9+beta 1抗体[Y9A2]
  • 描述
    小鼠单克隆抗体[Y9A2] to Integrin alpha 9+beta 1
  • 特异性
    Ab27947 recognises alpha9 integrin expressed as a heterodimer in association with the beta1 integrin.
  • 经测试应用
    适用于: ICC/IF, Flow Cyt, IP, WB, ICCmore details
    不适用于: IHC-FoFr,IHC-P or IHC-R
  • 种属反应性
    与反应: Rat, Guinea pig, Human
  • 免疫原

    Tissue/ cell preparation (Human): alpha9 integrin transfected lambda cells.

  • 常规说明


    This clone (Y9A2) inhibits adhesion of alpha9 expressing cells to N terminal osteopontin.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • 存储溶液
    Preservative: 0.09% Sodium Azide.
    Constituents: PBS, pH 7.4.
  • Concentration information loading...
  • 纯度
    Protein G purified
  • 纯化说明
    Ab27947 was purified by affinity chromatography, from tissue culture supernatant.
  • Primary antibody说明
    This clone (Y9A2) inhibits adhesion of alpha9 expressing cells to N terminal osteopontin.
  • 克隆
    单克隆
  • 克隆编号
    Y9A2
  • 骨髓瘤
    Sp2/0
  • 同种型
    IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab27947 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration. PubMed: 20810886
Flow Cyt Use 10µl for 106 cells.

(in 100µl). ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. PubMed: 20810886
ICC Use at an assay dependent concentration.
  • 应用说明
    Is unsuitable for IHC-FoFr,IHC-P or IHC-R.
  • 靶标

    • 相关性
      Integrins are heterodimeric proteins made up of alpha and beta subunits. At least 18 alpha and 8 beta subunits have been described in mammals. Integrin family members are membrane receptors involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metatastatic diffusion of tumor cells. Integrin alpha 9, when bound to the integrin beta 1 chain, forms an integrin that is a receptor for VCAM1, cytotactin and osteopontin. Expression of integrin alpha 9 has been found to be upregulated in small cell lung cancers.
    • 细胞定位
      Type I membrane protein.
    • 数据库链接
    • 别名
      • CD29 antibody
      • FNRB antibody
      • GPIIA antibody
      • ITGA9 antibody
      • ITGB1 antibody
      • MDF2 antibody
      • MSK12 antibody
      • VLAB antibody
      see all

    Anti-Integrin alpha 9+beta 1 antibody [Y9A2] (ab27947)参考文献

    This product has been referenced in:
    • Ou J  et al. Fibronectin extra domain A (EDA) sustains CD133(+)/CD44(+) subpopulation of colorectal cancer cells. Stem Cell Res 11:820-33 (2013). Read more (PubMed: 23811539) »
    • Eva R  et al. Rab11 and its effector Rab coupling protein contribute to the trafficking of beta 1 integrins during axon growth in adult dorsal root ganglion neurons and PC12 cells. J Neurosci 30:11654-69 (2010). ICC/IF, Inhibition Assay ; Rat . Read more (PubMed: 20810886) »

    See all 5 Publications for this product

    Product Wall

    Thank you for your enquiry.

    The following information is taken from Human Protein Atlas.

    1) Integrin alpha 9 positive control: Digestive tract
    http://www.proteinatlas.org/ENSG00000144668

    2) Integrin beta 1 positive...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunocytochemistry
    Sample
    Human Cultured Cells (Endothelial cells and fibroblasts cultured from hu)
    Specification
    Endothelial cells and fibroblasts cultured from hu
    Fixative
    Methanol
    Blocking step
    Serum as blocking agent for 5 minute(s) · Concentration: 10% · Temperature: 25°C
    Username

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    Verified customer

    提交于 Sep 16 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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