Anti-Integrin alpha 6抗体[MP 4F10] (ab20142)

概述

  • 产品名称Anti-Integrin alpha 6抗体[MP 4F10]
    参阅全部 Integrin alpha 6 一抗
  • 描述
    小鼠单克隆抗体[MP 4F10] to Integrin alpha 6
  • 经测试应用适用于: IHC-P, IHC-Fr, Flow Cyt, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Cell preparation: SW1222 human colorectal cell line.

  • 阳性对照
    • Human lymph node tissue.

性能

应用

Our Abpromise guarantee covers the use of ab20142 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/50. (from PubMed:17370299)
IHC-Fr Use at an assay dependent concentration.
Flow Cyt Use 1µg for 106 cells. (paraformaldehyde or methanol fixed cells)



ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
ICC/IF Use a concentration of 1 µg/ml.

靶标

  • 功能Integrin alpha-6/beta-1 is a receptor for laminin on platelets. Integrin alpha-6/beta-4 is a receptor for laminin in epithelial cells and it plays a critical structural role in the hemidesmosome (By similarity). ITGA6:ITGB4 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling (PubMed:20682778). ITGA6:ITGB4 binds to IGF1 and this binding is essential for IGF1 signaling (PubMed:22351760).
  • 组织特异性Integrin alpha-6/beta-4 is predominantly expressed by epithelia. Isoforms containing segment X1 are ubiquitously expressed. Isoforms containing segment X1X2 are expressed in heart, kidney, placenta, colon, duodenum, myoblasts and myotubes, and in a limited number of cell lines; they are always coexpressed with the ubiquitous isoform containing segment X1. In some tissues (e.g. Salivary gland), isoforms containing cytoplasmic segment A and isoforms containing segment B are detected while in others, only isoforms containing one cytoplasmic segment are found (segment A in epidermis and segment B in kidney).
  • 疾病相关Epidermolysis bullosa letalis, with pyloric atresia
  • 序列相似性Belongs to the integrin alpha chain family.
    Contains 7 FG-GAP repeats.
  • 翻译后修饰Isoforms containing segment A, but not segment B, are the major targets for PMA-induced phosphorylation. Phosphorylation occurs on 'Ser-1103' of isoform alpha-6X1X2A. Phosphorylation is not required for the induction of integrin alpha-6A/beta-1 high affinity but may reduce the affinity for ligand.
    In invasive prostate cancer ITGA6 undergoes PLAU-mediated cleavage at residues Arg-634-635-Arg in a time-dependent manner enhancing cell invasion and migration in vitro.
    Palmitoylation by DHHC3 enhances stability and cell surface expression.
  • 细胞定位Cell membrane. Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Alpha6p antibody
    • CD49 antigen-like family member F antibody
    • CD49f antibody
    • Integrin alpha6B antibody
    • Integrin, alpha 6 antibody
    • ITA6_HUMAN antibody
    • ITGA6 antibody
    • ITGA6B antibody
    • Processed integrin alpha-6 antibody
    • VLA 6 antibody
    • VLA-6 antibody
    see all

Anti-Integrin alpha 6 antibody [MP 4F10] 图像

  • ab29871) by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded tissue sections). The sections were fixed in paraformaldehyde prior to blocking with 10% serum for 1 hour at 20°C. The primary antibody was diluted 1/400 and incubated with the sample for 12 hour at 4°C. An HRP-conjugated rabbit anti-mouse polyclonal was used as the secondary antibody, diluted 1/200.

    See Abreview

  • ICC/IF image of ab150035). The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M (ab7481) glycine (ab120050) in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab150113) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Overlay histogram showing HT1080 cells stained with 6 cells) for 30 min at 22°C. The secondary antibody used was a goat anti-mouse DyLight® 488 (IgG; H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HT1080 cells fixed with methanol (5 min) used under the same conditions.

    Please note that Abcam does not have data for use of this antibody on non-fixed cells. We welcome any customer feedback.

Anti-Integrin alpha 6 antibody [MP 4F10] (ab20142)参考文献

This product has been referenced in:
  • Svensson Månsson S  et al. Transcriptional upregulation and unmethylation of the promoter region of p16 in invasive basal cell carcinoma cells and partial co-localization with the gamma 2 chain of laminin-332. J Pathol 212:102-11 (2007). Read more (PubMed: 17370299) »
  • Levy L  et al. beta1 integrins regulate keratinocyte adhesion and differentiation by distinct mechanisms. Mol Biol Cell 11:453-66 (2000). Read more (PubMed: 10679006) »

See all 3 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step 10% goat serum diluted in 2%BSA/PBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Antigen retrieval step Other
Sample Human Tissue sections (Skin)
Specification Skin
Permeabilization No
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Apr 03 2014

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 27°C
Sample Human Cell (HUVEC)
Specification HUVEC
Permeabilization Yes - 0.1% Triton
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Jan 02 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HUVEC)
Specification HUVEC
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 1°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Sep 17 2013

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Liver)
Specification Liver
Fixative Paraformaldehyde
Antigen retrieval step None - Buffer/Enzyme Used: Citrate 0,01M pH6
Permeabilization No
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Feb 19 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (skin)
Specification skin
Fixative Paraformaldehyde
Permeabilization Yes - 0.2% Trititon X-100
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Dr. Michael Bachelor

Verified customer

提交于 Jul 18 2012

Thank you for contacting Abcam.

I am sorry that you are having some issues with ab20142well, the only image we have of it being used in IHC-P is from an Abreview and that information can be found below:

http://www.abcam.com/index.ht...

Read More

Thank you very much for contacting us - and sending the enquiry as a table. That clear overview was very helpful :-) As you may know, we have not tested the individual samples for their expression of the mentioned targets. However, according to the ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (Skin)
Specification Skin
Fixative Acetone
Permeabilization No
Blocking step Casein as blocking agent for 10 minute(s) · Concentration: 0.25% · Temperature: 20°C
Username

Dr. Vaughan Feisst

Verified customer

提交于 Jul 08 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Lymph node)
Specification Lymph node
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 May 31 2011

Thank you for your enquiry regarding our Anti-Integrin a6 Antibodies. The antibodies ab19765 and ab20142 come from different sources and therefore the decision not to test ab19765 in IHC-P comes only from the fact that the laboratory source of this an...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"