Anti-Integrin alpha 6抗体[GoH3] (Phycoerythrin) (ab95703)


  • 产品名称Anti-Integrin alpha 6抗体[GoH3] (Phycoerythrin)
    参阅全部 Integrin alpha 6 一抗
  • 描述
    大鼠单克隆抗体[GoH3] to Integrin alpha 6 (Phycoerythrin)
  • 偶联物Phycoerythrin. Ex: 488nm, Em: 575nm
  • 经测试应用适用于: Flow Cyt, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Cow, Human
  • 免疫原

    Mouse mammary tumor cells

  • 阳性对照
    • Normal Human peripheral blood cells/leukocytes
  • 常规说明

    The GoH3 antibody has been reported to have functional activity in blocking the binding of Integrin alpha 6 to laminin.



Our Abpromise guarantee covers the use of ab95703 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use 0.06-0.125µg for 105-8 cells. Staining the cell sample in a final volume of 100 µl is recommended.

ab134674-Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
ICC/IF Use at an assay dependent concentration. PubMed: 23613946


  • 功能Integrin alpha-6/beta-1 is a receptor for laminin on platelets. Integrin alpha-6/beta-4 is a receptor for laminin in epithelial cells and it plays a critical structural role in the hemidesmosome (By similarity). ITGA6:ITGB4 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling (PubMed:20682778). ITGA6:ITGB4 binds to IGF1 and this binding is essential for IGF1 signaling (PubMed:22351760).
  • 组织特异性Integrin alpha-6/beta-4 is predominantly expressed by epithelia. Isoforms containing segment X1 are ubiquitously expressed. Isoforms containing segment X1X2 are expressed in heart, kidney, placenta, colon, duodenum, myoblasts and myotubes, and in a limited number of cell lines; they are always coexpressed with the ubiquitous isoform containing segment X1. In some tissues (e.g. Salivary gland), isoforms containing cytoplasmic segment A and isoforms containing segment B are detected while in others, only isoforms containing one cytoplasmic segment are found (segment A in epidermis and segment B in kidney).
  • 疾病相关Epidermolysis bullosa letalis, with pyloric atresia
  • 序列相似性Belongs to the integrin alpha chain family.
    Contains 7 FG-GAP repeats.
  • 翻译后修饰Isoforms containing segment A, but not segment B, are the major targets for PMA-induced phosphorylation. Phosphorylation occurs on 'Ser-1103' of isoform alpha-6X1X2A. Phosphorylation is not required for the induction of integrin alpha-6A/beta-1 high affinity but may reduce the affinity for ligand.
    In invasive prostate cancer ITGA6 undergoes PLAU-mediated cleavage at residues Arg-634-635-Arg in a time-dependent manner enhancing cell invasion and migration in vitro.
    Palmitoylation by DHHC3 enhances stability and cell surface expression.
  • 细胞定位Cell membrane. Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Alpha6p antibody
    • CD49 antigen-like family member F antibody
    • CD49f antibody
    • Integrin alpha6B antibody
    • Integrin, alpha 6 antibody
    • ITA6_HUMAN antibody
    • ITGA6 antibody
    • ITGA6B antibody
    • Processed integrin alpha-6 antibody
    • VLA 6 antibody
    • VLA-6 antibody
    see all

Anti-Integrin alpha 6 antibody [GoH3] (Phycoerythrin) 图像

  • Flow cytometry staining of normal Human peripheral blood cells with 0.06 µg of Rat IgG2a ? Isotype Control PE (open histogram) or 0.06 µg of ab95703 (filled histogram). Cells in the lymphocyte (left) and granulocyte (right) gates were used for analysis.
  • Flow Cytometry analysis of normal human peripheral blood cells stained with Rat IgG2a kappa Isotype Control Phycoerythrin (blue histogram) or Anti-Integrin alpha 6 antibody [GoH3] (Phycoerythrin) (ab95703) (purple histogram). Cells in the lymphocyte gate were used for analysis.

Anti-Integrin alpha 6 antibody [GoH3] (Phycoerythrin) (ab95703)参考文献

This product has been referenced in:
  • Ngamjariyawat A  et al. Co-culture of neural crest stem cells (NCSC) and insulin producing beta-TC6 cells results in cadherin junctions and protection against cytokine-induced beta-cell death. PLoS One 8:e61828 (2013). ICC/IF ; Mouse . Read more (PubMed: 23613946) »
  • Ambrose HE & Wagner SD Alpha6-integrin is expressed on germinal centre B cells and modifies growth of a B-cell line. Immunology 111:400-6 (2004). Flow Cyt . Read more (PubMed: 15056376) »

See all 3 Publications for this product

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For ab95703 (Anti-Human/Mouse CD49f (Integrin alpha 6) PE), it is a cell surface staining antibody. We test this antibody in-house as a cell surface stain on normal h...

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