Anti-Integrin alpha 5抗体[SAM-1] (ab6131)

概述

  • 产品名称Anti-Integrin alpha 5抗体[SAM-1]
    参阅全部 Integrin alpha 5 一抗
  • 描述
    小鼠单克隆抗体[SAM-1] to Integrin alpha 5
  • 特异性NKI-SAM-1 recognizes the 135-kDa integrin- alpha5 monocyte-specific antigen. As a result NKI-SAM-1 strongly reacts with monocytes, and weakly with granulocytes, platelets and T-lymphocytes. Its exact epitope is not known.
  • 经测试应用适用于: Flow Cyt, IP, ICC/IF, IHC-Frmore details
  • 种属反应性
    与反应: Human, Macaque Monkey
  • 免疫原

    Integrin alpha 5 positive cell preparation.

  • 常规说明

    Integrin alpha 5 is a heterodimer that associates noncovalently with CD29/integrin beta 1 subunit to form the alpha-5-beta-1 very late antigen (VLA-5) complex. VLA-5 is a fibronectin receptor that is expressed on thymocytes, T-cells, monocytes and platelets. It is also found on very early B-cells and activated B-cells. VLA-5-mediated binding to fibronectin sends a costimulatory signal to T-cells and enhances Fc-gamma-R- and complement receptor-mediated phago-cytosis. It is also involved in monocyte migration into extracellular tissues.

    NKI-SAM-1 is a monoclonal antibody obtained by fusion of mouse myeloma cells with spleen cells from BALB/C mice immunized with U937 histiocytic lymphoma cells.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: 0.1% Sodium Azide
    Constituents: PBS (pH 7.4)
  • Concentration information loading...
  • 纯度Protein G purified
  • Primary antibody说明Integrin alpha 5 is a heterodimer that associates noncovalently with CD29/integrin beta 1 subunit to form the alpha-5-beta-1 very late antigen (VLA-5) complex. VLA-5 is a fibronectin receptor that is expressed on thymocytes, T-cells, monocytes and platelets. It is also found on very early B-cells and activated B-cells. VLA-5-mediated binding to fibronectin sends a costimulatory signal to T-cells and enhances Fc-gamma-R- and complement receptor-mediated phago-cytosis. It is also involved in monocyte migration into extracellular tissues.
  • 克隆单克隆
  • 克隆编号SAM-1
  • 同种型IgG2b
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab6131 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use 0.2-0.5µg for 106 cells.
IP Use a concentration of 0.5 - 2 µg/ml.
ICC/IF 1/200. (see Abreview).
IHC-Fr Use at an assay dependent concentration.

靶标

  • 功能Integrin alpha-5/beta-1 is a receptor for fibronectin and fibrinogen. It recognizes the sequence R-G-D in its ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
  • 序列相似性Belongs to the integrin alpha chain family.
    Contains 7 FG-GAP repeats.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CD49 antigen-like family member E antibody
    • CD49e antibody
    • Fibronectin receptor subunit alpha antibody
    • Fibronectin receptor, alpha subunit antibody
    • FNRA antibody
    • Integrin alpha 5 (fibronectin receptor alpha) antibody
    • Integrin alpha-5 antibody
    • Integrin alpha-5 light chain antibody
    • Integrin alpha-F antibody
    • Integrin, alpha 5 (fibronectin receptor, alpha polypeptide) antibody
    • ITA5_HUMAN antibody
    • Itga5 antibody
    • Very late activation protein 5, alpha subunit antibody
    • VLA-5 antibody
    • VLA5 antibody
    • VLA5A antibody
    see all

Anti-Integrin alpha 5 antibody [SAM-1] 图像

  • ab6131 at 1/200 staining human retinal epithelial cells by ICC/IF. The cells were permeabilized with 100% methanol and blocked with 1%BSA / 4% goat serum prior to incubation with the antibody for 30 minutes. An Alexa-Fluor ® 488 conjugated goat anti-rabbit IgG (H+L) was used as the secondary.

    See Abreview

  • ab6131 staining Integrin alpha 5 in Human synovial fibroblasts by Flow Cytometry. Cells were detached by accutase, spun and fixed in 1,5 % formalin for 10 min.  Gating was done on synovial fibroblasts. The  sample was incubated with the primary antibody, dilution 1/500 (1% BSA in PBS), for 20 minutes at 25°C.  A PE-conjugated Goat polyclonal to mouse IgG, dilution 1/200, was used as secondary antibody. The flow cytometry plot is an overlay of the isotype control ( IgG2b, red peak) with the sample (white peak); (equal amounts of isotype IgG2b and antibody were used).

    See Abreview

Anti-Integrin alpha 5 antibody [SAM-1] (ab6131)参考文献

This product has been referenced in:
  • Tartaglia LJ  et al. Molecular cloning, overexpression, and an efficient one-step purification of a5ß1 integrin. Protein Expr Purif 92:21-8 (2013). Read more (PubMed: 23993979) »
  • Rowland TJ  et al. Roles of integrins in human induced pluripotent stem cell growth on Matrigel and vitronectin. Stem Cells Dev 19:1231-40 (2010). ICC/IF ; Human . Read more (PubMed: 19811096) »

See all 2 Publications for this product

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Thank you for your enquiry.

I apologise for the delay in getting back to you. I have been revewing and checking the immunogen sequences of our Integrin alpha 5 antibody. Regrettably, so far I am sorry I have not found one that would match yo...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Human Cell (synovial fibroblasts)
Specification synovial fibroblasts
Fixation Paraformaldehyde
Permeabilization No
Gating Strategy only synovial fibroblasts
Username

Dr. Torsten Lowin

Verified customer

提交于 Aug 13 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Renal Epithelial Cell)
Specification Renal Epithelial Cell
Fixative Methanol
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 4%
Username

Abcam user community

Verified customer

提交于 Aug 10 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"