Anti-Integrin alpha 5抗体[P1D6] (ab78614)


  • 产品名称
    Anti-Integrin alpha 5抗体[P1D6]
    参阅全部 Integrin alpha 5 一抗
  • 描述
    小鼠单克隆抗体[P1D6] to Integrin alpha 5
  • 特异性
    ab78614 is not reactive with the denatured Integrin alpha 5 subunit.
  • 经测试应用
    适用于: Dot blot, IHC-Fr, Flow Cyt, Inhibition Assay, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Tissue, cells or virus corresponding to Human Integrin alpha 5.
    Database link: P08648

  • 阳性对照
    • Ovary. HeLa cells. Widely expressed



Our Abpromise guarantee covers the use of ab78614 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Dot blot Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
Flow Cyt Use 1µg for 106 cells. ab91537-Mouse monoclonal IgG3, is suitable for use as an isotype control with this antibody.
Inhibition Assay Use at an assay dependent concentration. Suitable for use in attachment inhibition assays using fibroblasts, endothelial cells, platelets and T and B lymphocytes on fibronectin.
ICC/IF 1/200.


  • 功能
    Integrin alpha-5/beta-1 is a receptor for fibronectin and fibrinogen. It recognizes the sequence R-G-D in its ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
  • 序列相似性
    Belongs to the integrin alpha chain family.
    Contains 7 FG-GAP repeats.
  • 细胞定位
  • Information by UniProt
  • 数据库链接
  • 别名
    • CD49 antigen-like family member E antibody
    • CD49e antibody
    • Fibronectin receptor subunit alpha antibody
    • Fibronectin receptor, alpha subunit antibody
    • FNRA antibody
    • Integrin alpha 5 (fibronectin receptor alpha) antibody
    • Integrin alpha-5 antibody
    • Integrin alpha-5 light chain antibody
    • Integrin alpha-F antibody
    • Integrin, alpha 5 (fibronectin receptor, alpha polypeptide) antibody
    • ITA5_HUMAN antibody
    • Itga5 antibody
    • Very late activation protein 5, alpha subunit antibody
    • VLA-5 antibody
    • VLA5 antibody
    • VLA5A antibody
    see all


  • Overlay histogram showing HeLa cells stained with ab78614 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine (ab120050) to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab78614, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG3 [MG3-35] (ab18394, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in 4% paraformaldehyde (10 min) fixed HeLa cells used under the same conditions.
    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

  • Flow Cytometry Analysis: Representative lot data.
    Cell surface staining of HT-1080 cells using 1 µg/mL of ab78614 (shaded histogram) and an isotype control (unshaded histogram). Cells were detected using a mouse IgG-FITC.



This product has been referenced in:
  • Lee P  et al. A novel cell adhesion region in tropoelastin mediates attachment to integrin aVß5. J Biol Chem 289:1467-77 (2014). Human . Read more (PubMed: 24293364) »
  • Mardilovich A  et al. Design of a novel fibronectin-mimetic peptide-amphiphile for functionalized biomaterials. Langmuir 22:3259-64 (2006). Inhibition Assay . Read more (PubMed: 16548586) »

See all 2 Publications for this product


I would recommend Anti-Integrin alpha 5 antibody [P1D6] (ab78614) which is the only anti-integrin alpha 5 antibody we list in our catalogue whose inhibitory activity has been previously tested. It is therefore suitable for use in attachment inhibi...

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Thank you for your enquiry.

I can confirm that this antibody does not contain azide and the storage buffer consists of 250 mM Sodium chloride, 20mM Sodium phosphate buffer, pH 7.6 which are not toxic. Thus, we do not have MSDS document for th...

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Thank you for your enquiry.

I apologise for the delay in getting back to you. I have been revewing and checking the immunogen sequences of our Integrin alpha 5 antibody. Regrettably, so far I am sorry I have not found one that would match yo...

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Immunocytochemistry/ Immunofluorescence
Human Cell (Human foreskin fibroblast)
Human foreskin fibroblast
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 20°C

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提交于 Oct 25 2011