The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 0.125-0.5µg for 105-8 cells.
Use 0.125 - 0.5 µg for 105 - 108 cells in a 100 µl volume.
相关性Integrin alpha 4 beta 7, also known as, the lymphocyte Peyer's patch adhesion molecule (LPAM1) is a member of the integrin family of cell surface receptors. It is expressed primarily on mucosal lymphocytes, but is also present on NK cells and eosinophils. The alpha 4 beta 7 heterodimer mediates the binding of lymphocytes to its ligand, mucosal vascular addressin (MAdCAM1) on the high endothelial venules, thereby directing the homing of lymphocytes into Peyer's patches and intestinal lamina propria.
细胞定位Membrane; Single pass type I membrane protein.
Staining of C57Bl/6 bone marrow cells with FITC anti-Mouse/Human B220 (RA3-6B2) and 0.125 µg of APC Rat IgG2a Isotype Control (left), or 0.125 µg of ab93539 (right). Total viable cells were used for analysis.
Andrew DP et al. Distinct but overlapping epitopes are involved in alpha 4 beta 7-mediated adhesion to vascular cell adhesion molecule-1, mucosal addressin-1, fibronectin, and lymphocyte aggregation. J Immunol153:3847-61 (1994).
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Hamann A et al. Role of alpha 4-integrins in lymphocyte homing to mucosal tissues in vivo. J Immunol152:3282-93 (1994).
Read more (PubMed: 7511642) »