Anti-Integrin alpha 4+beta 7抗体[DATK32] (ab25329)

概述

  • 产品名称Anti-Integrin alpha 4+beta 7抗体[DATK32]
    参阅全部 Integrin alpha 4+beta 7 一抗
  • 描述
    大鼠单克隆抗体[DATK32] to Integrin alpha 4+beta 7
  • 特异性This antibody is specific to mouse LPAM1 (Integrin alpha 4 beta 7).
  • 经测试应用适用于: Flow Cyt, Blocking, IHC-Frmore details
  • 种属反应性
    与反应: Mouse
  • 免疫原

    Tissue/ cell preparation: AKR/Cum mouse spontaneous T lymphoma cell line TK1.

  • 表位This antibody recognises a conformational epitope on the heterodimer.
  • 常规说明

    This antibody has been shown to be useful for studies of the induction of homotypic cellular aggregation and the blockade of binding to cell adhesion molecules.

性能

应用

Our Abpromise guarantee covers the use of ab25329 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use 1µg for 106 cells. ab18450-Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
Blocking Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

靶标

  • 相关性Integrin alpha 4 beta 7, also known as, the lymphocyte Peyer's patch adhesion molecule (LPAM1) is a member of the integrin family of cell surface receptors. It is expressed primarily on mucosal lymphocytes, but is also present on NK cells and eosinophils. The alpha 4 beta 7 heterodimer mediates the binding of lymphocytes to its ligand, mucosal vascular addressin (MAdCAM1) on the high endothelial venules, thereby directing the homing of lymphocytes into Peyer's patches and intestinal lamina propria.
  • 细胞定位Membrane; Single pass type I membrane protein.
  • 数据库链接
  • 别名
    • Antigen CD49D antibody
    • CD49d antibody
    • IA4 antibody
    • Integrin alpha 4 subunit antibody
    • Integrin alpha IV antibody
    • Integrin beta 7 antibody
    • Integrin beta 7 subunit antibody
    • ITGA4 antibody
    • ITGB7 antibody
    • LPAM1 antibody
    see all

Anti-Integrin alpha 4+beta 7 antibody [DATK32] 图像

  • Flow Cytometry analysis of BALB/c mouse splenocytes labeling Integrin alpha 4+beta 7 with ab25329 at 1 μg/106 cells (purple). A Mouse Anti-Rat IgG2a-CY5 was used as the secondary antibody. Grey - Isotype Control, Rat IgG2a-UNLB, followed by Mouse Anti-Rat IgG2a-CY5.

  • Ab25329 at 1µg pre 106 cells positively staining LPAM1 in the TK1 cell line. The control or negative cells are from the 8-81 MuLV transformed preB cell line. After labelling with Ab25329, the cells were stained with goat anti rat IgG FITC.

Anti-Integrin alpha 4+beta 7 antibody [DATK32] (ab25329)参考文献

ab25329 has not yet been referenced specifically in any publications.

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