Anti-Integrin alpha 4抗体[P1H4] (ab78964)


  • 产品名称Anti-Integrin alpha 4抗体[P1H4]
    参阅全部 Integrin alpha 4 一抗
  • 描述
    小鼠单克隆抗体[P1H4] to Integrin alpha 4
  • 经测试应用适用于: IP, ELISA, RIA, ICC, Flow Cyt, Inhibition Assay, IHC-Frmore details
  • 种属反应性
    与反应: Human
    预测可用于: Non Human Primates
  • 免疫原

    Human T lymphocytes

  • 阳性对照
    • Intestinal mucosa.



Our Abpromise guarantee covers the use of ab78964 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use at an assay dependent dilution.
ELISA Use at an assay dependent dilution.
RIA Use at an assay dependent dilution.
ICC Use at an assay dependent dilution.
Flow Cyt Use 1µg for 106 cells.
Inhibition Assay Use at an assay dependent dilution. ab78964 inhibits CS-1 and VCAM binding.
IHC-Fr Use at an assay dependent dilution. Preferred fixatives are acetone and alcohols.


  • 功能Integrins alpha-4/beta-1 (VLA-4) and alpha-4/beta-7 are receptors for fibronectin. They recognize one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. They are also receptors for VCAM1. Integrin alpha-4/beta-1 recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-4/beta-7 is also a receptor for MADCAM1. It recognizes the sequence L-D-T in MADCAM1. On activated endothelial cells integrin VLA-4 triggers homotypic aggregation for most VLA-4-positive leukocyte cell lines. It may also participate in cytolytic T-cell interactions with target cells.
  • 序列相似性Belongs to the integrin alpha chain family.
    Contains 7 FG-GAP repeats.
  • 结构域The SG1 motif is involved in binding to chondroitin sulfate glycosaminoglycan and cell adhesion.
  • 翻译后修饰Phosphorylation on Ser-1027 inhibits PXN binding.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 269C wild type antibody
    • Antigen CD49D, alpha 4 subunit of VLA 4 receptor antibody
    • CD49 antigen like family member D antibody
    • CD49 antigen-like family member D antibody
    • CD49d antibody
    • IA4 antibody
    • Integrin alpha 4 antibody
    • Integrin alpha 4 subunit antibody
    • Integrin alpha IV antibody
    • Integrin alpha-4 antibody
    • Integrin alpha-IV antibody
    • Integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA 4 receptor) antibody
    • ITA4_HUMAN antibody
    • ITGA4 antibody
    • MGC90518 antibody
    • OTTHUMP00000205320 antibody
    • Very late activation protein 4 receptor, alpha 4 subunit antibody
    • VLA 4 subunit alpha antibody
    • VLA-4 subunit alpha antibody
    • VLA4 antibody
    see all

Anti-Integrin alpha 4 antibody [P1H4] 图像

  • Overlay histogram showing Jurkat cells stained with ab78964 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab78964, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in 80% methanol (5 min) fixed Jurkat cells used under the same conditions.
    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

Anti-Integrin alpha 4 antibody [P1H4] (ab78964)参考文献

This product has been referenced in:
  • Zhang L  et al. Correlation between tumor suppressor inhibitor of growth family member 4 expression and microvessel density in breast cancer. Hum Pathol 43:1611-7 (2012). WB . Read more (PubMed: 22436625) »
  • Legrand F  et al. Human Eosinophils Exert TNF-{alpha} and Granzyme A-Mediated Tumoricidal Activity toward Colon Carcinoma Cells. J Immunol 185:7443-51 (2010). Inhibition Assay . Read more (PubMed: 21068403) »

See all 2 Publications for this product

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We do not provide expiry dates as such. For antibodies, we recommend they can be stored up to 1 year after delivery under the storage conditions stated on the datasheet.

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