使用敲除细胞株进行验证RabMAb

Anti-Integrin alpha 4抗体[EPR1355Y] (ab81280)

概述

  • 产品名称Anti-Integrin alpha 4抗体[EPR1355Y]
    参阅全部 Integrin alpha 4 一抗
  • 描述
    兔单克隆抗体[EPR1355Y] to Integrin alpha 4
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Integrin alpha 4 aa 1000-1100 (C terminal).

  • 阳性对照
    • Jurkat cell lysate
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A trial size is available to purchase for this antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

应用

Our Abpromise guarantee covers the use of ab81280 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/2000. Predicted molecular weight: 150 kDa.

靶标

  • 功能Integrins alpha-4/beta-1 (VLA-4) and alpha-4/beta-7 are receptors for fibronectin. They recognize one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. They are also receptors for VCAM1. Integrin alpha-4/beta-1 recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-4/beta-7 is also a receptor for MADCAM1. It recognizes the sequence L-D-T in MADCAM1. On activated endothelial cells integrin VLA-4 triggers homotypic aggregation for most VLA-4-positive leukocyte cell lines. It may also participate in cytolytic T-cell interactions with target cells.
  • 序列相似性Belongs to the integrin alpha chain family.
    Contains 7 FG-GAP repeats.
  • 结构域The SG1 motif is involved in binding to chondroitin sulfate glycosaminoglycan and cell adhesion.
  • 翻译后修饰Phosphorylation on Ser-1027 inhibits PXN binding.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 269C wild type antibody
    • Antigen CD49D, alpha 4 subunit of VLA 4 receptor antibody
    • CD49 antigen like family member D antibody
    • CD49 antigen-like family member D antibody
    • CD49d antibody
    • IA4 antibody
    • Integrin alpha 4 antibody
    • Integrin alpha 4 subunit antibody
    • Integrin alpha IV antibody
    • Integrin alpha-4 antibody
    • Integrin alpha-IV antibody
    • Integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA 4 receptor) antibody
    • ITA4_HUMAN antibody
    • ITGA4 antibody
    • MGC90518 antibody
    • OTTHUMP00000205320 antibody
    • Very late activation protein 4 receptor, alpha 4 subunit antibody
    • VLA 4 subunit alpha antibody
    • VLA-4 subunit alpha antibody
    • VLA4 antibody
    see all

Anti-Integrin alpha 4 antibody [EPR1355Y] 图像



  • Predicted band size : 150 kDa

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: Integrin alpha 4 knockout HAP1 cell lysate (20 µg)
    Lanes 1 and 2: Merged signal (red and green). Green - ab81280 observed at 150 kDa. Red - loading control, ab8226, observed at 42 kDa.
    ab81280 was shown to recognize Integrin alpha 4 when Integrin alpha 4 knockout samples were used, along with additional cross-reactive bands. Wild-type and Integrin alpha 4 knockout samples were subjected to SDS-PAGE. ab81280 and ab8226 (loading control to beta actin) were diluted 1/2000 and 1/1000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) ab216776 secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

  • Anti-Integrin alpha 4 antibody [EPR1355Y] (ab81280) at 1/2000 dilution + Jurkat cell lysate at 10 µg

    Secondary
    HRP labelled goat anti rabbit at 1/2000 dilution

    Predicted band size : 150 kDa
    Observed band size : 150 kDa

Anti-Integrin alpha 4 antibody [EPR1355Y] (ab81280)参考文献

This product has been referenced in:
  • de la Rica L  et al. NF-?B-direct activation of microRNAs with repressive effects on monocyte-specific genes is critical for osteoclast differentiation. Genome Biol 16:2 (2015). WB . Read more (PubMed: 25601191) »
  • Buggins AG  et al. Evidence for a macromolecular complex in poor prognosis CLL that contains CD38, CD49d, CD44 and MMP-9. Br J Haematol 154:216-22 (2011). IF, WB ; Human . Read more (PubMed: 21569005) »

See all 3 Publications for this product

Product Wall

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The immunogen for ab81280 corresponds to an intracellular region of the protein:

http://www.uniprot.org/blast/?about=P13612[1002-1032]

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"