Anti-Integrin alpha 2+beta 1抗体[P1E6] (ab78883)

概述

  • 产品名称Anti-Integrin alpha 2+beta 1抗体[P1E6]
    参阅全部 Integrin alpha 2+beta 1 一抗
  • 描述
    小鼠单克隆抗体[P1E6] to Integrin alpha 2+beta 1
  • 经测试应用适用于: Flow Cyt, Inhibition Assay, ICC/IFmore details
    不适用于: IHC-P
  • 种属反应性
    与反应: Human
  • 免疫原

    Tissue/ cell preparation : Human HT1080, whole cells.

  • 阳性对照
    • Skin (basement membrane) and HepG2 cell line (IF/ICC)

性能

应用

Our Abpromise guarantee covers the use of ab78883 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
Inhibition Assay Use at an assay dependent concentration. Suitable for use with fibroblasts, epithelial cells, endothelial cells, and non activated platelets on collagen types I, III, IV, VI and laminin.
ICC/IF Use at an assay dependent concentration. Suitable for use with fresh frozen or acetone fixed tissues or cell culture.
  • 应用说明Is unsuitable for IHC-P.
  • 靶标

    • 细胞定位Cell Membrane; single-pass type I membrane protein
    • 数据库链接
    • 别名
      • CD29 antibody
      • CD49B antibody
      • Collagen receptor antibody
      • GPIa antibody
      • GPIIA antibody
      • Integrin alpha 2 antibody
      • Integrin beta 1 antibody
      • ITGA2 antibody
      • ITGB1 antibody
      • Platelet antigen Br antibody
      • Platelet glycoprotein GPIa antibody
      • Platelet glycoprotein Ia antibody
      • Platelet membrane glycoprotein Ia antibody
      • Very late activation protein 2 receptor alpha 2 subunit antibody
      • VLA 2 alpha chain antibody
      • VLA 2 antibody
      • VLA4 antibody
      • VLAA2 antibody
      see all

    Anti-Integrin alpha 2+beta 1 antibody [P1E6] 图像

    • ICC/IF image of ab78883 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab78883, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% formaldehyde fixed (10 min) MCF7 cells at 5µg/ml.
    • Overlay histogram showing HT1080 cells stained with ab78883 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab78883, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in 80% methanol (5 min) fixed HT1080 cells used under the same conditions.

    Anti-Integrin alpha 2+beta 1 antibody [P1E6] (ab78883)参考文献

    ab78883 has not yet been referenced specifically in any publications.

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