使用敲除细胞株进行验证RabMAb

Anti-Integrin alpha 2抗体[EPR5789] (ab109432)

概述

  • 产品名称Anti-Integrin alpha 2抗体[EPR5789]
    参阅全部 Integrin alpha 2 一抗
  • 描述
    兔单克隆抗体[EPR5789] to Integrin alpha 2
  • 经测试应用适用于: WBmore details
    不适用于: Flow Cyt,IHC-P or IP
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to residues near the C-terminus of Human Integrin alpha 2.

  • 阳性对照
    • 293T, T47-D, and A431 cell lysates
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A trial size is available to purchase for this antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

应用

Our Abpromise guarantee covers the use of ab109432 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Predicted molecular weight: 129 kDa.
  • 应用说明Is unsuitable for Flow Cyt,IHC-P or IP.
  • 靶标

    • 功能Integrin alpha-2/beta-1 is a receptor for laminin, collagen, collagen C-propeptides, fibronectin and E-cadherin. It recognizes the proline-hydroxylated sequence G-F-P-G-E-R in collagen. It is responsible for adhesion of platelets and other cells to collagens, modulation of collagen and collagenase gene expression, force generation and organization of newly synthesized extracellular matrix.
    • 序列相似性Belongs to the integrin alpha chain family.
      Contains 7 FG-GAP repeats.
      Contains 1 VWFA domain.
    • 结构域The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.
    • 细胞定位Membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • BR antibody
      • CD 49b antibody
      • CD49 antigen like family member B antibody
      • CD49 antigen-like family member B antibody
      • CD49b antibody
      • CD49b antigen antibody
      • Collagen receptor antibody
      • DX5 antibody
      • Glycoprotein Ia deficiency included antibody
      • GP Ia antibody
      • GP Ia deficiency, included antibody
      • GPIa antibody
      • HPA 5 included antibody
      • HPA5 included antibody
      • Human platelet alloantigen system 5 antibody
      • Integrin alpha 2 antibody
      • Integrin alpha-2 antibody
      • Integrin, alpha 2 (CD49B alpha 2 subunit of VLA 2 receptor) antibody
      • ITA2_HUMAN antibody
      • ITGA2 antibody
      • Platelet alloantigen Br(a), included antibody
      • Platelet antigen Br antibody
      • Platelet glycoprotein GPIa antibody
      • Platelet glycoprotein Ia antibody
      • Platelet glycoprotein Ia/IIa antibody
      • Platelet membrane glycoprotein Ia antibody
      • Platelet receptor for collagen, deficiency of, included antibody
      • Very late activation protein 2 receptor alpha 2 subunit antibody
      • VLA 2 alpha chain antibody
      • VLA 2 antibody
      • VLA 2 subunit alpha antibody
      • VLA-2 subunit alpha antibody
      • VLA2 antibody
      • VLA2 receptor alpha 2 subunit antibody
      • VLAA2 antibody
      see all

    Anti-Integrin alpha 2 antibody [EPR5789] 图像



    • Predicted band size : 129 kDa

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: Integrin alpha 2 knockout HAP1 cell lysate (20 µg)

      Lane 3: A431 cell lysate (20 µg)
      Lane 4: T47D cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab109432 observed at 165 kDa. Red - loading control, ab8245, observed at 37 kDa.
      ab109432 was shown to specifically react with Integrin alpha 2 when Integrin alpha 2 knockout samples were used. Wild-type and Integrin alpha 2 knockout samples were subjected to SDS-PAGE. ab109432 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

    • All lanes : Anti-Integrin alpha 2 antibody [EPR5789] (ab109432) at 1/1000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : T47-D cell lysate
      Lane 3 : A431 cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 129 kDa

    Anti-Integrin alpha 2 antibody [EPR5789] (ab109432)参考文献

    This product has been referenced in:
    • Guo F  et al. Gingival fibroblasts display reduced adhesion and spreading on extracellular matrix: a possible basis for scarless tissue repair? PLoS One 6:e27097 (2011). WB ; Human . Read more (PubMed: 22073262) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"