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Synthetic phosphopeptide derived from human Insulin Receptor around the phosphorylation site of tyrosine 1361 (I-P-YP-T-H).
Our Abpromise guarantee covers the use of ab60946 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|WB||1/500 - 1/1000. Detects a band of approximately 90 kDa (predicted molecular weight: 156 kDa).|
|ICC/IF||Use at an assay dependent concentration.|
ab60946 (1:10000) antibody detects endogenous levels of Insulin Receptor only when phosphorylated at Tyr1361.
ab60946 staining the insulin receptor in Human white blood cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with Triton X-100 (0.1%) and blocked with 2% serum for 1 hour at 25°C. Samples were incubated with primary antibody (1/500 in PBS + 2% BSA) for 12 hours at 4°C. An Alexa Fluor®488-conjugated Goat anti-rabbit IgG polyclonal (ab150077) (1/500) was used as the secondary antibody.