Anti-Insulin Receptor beta抗体[C18C4] (ab69508)


  • 产品名称Anti-Insulin Receptor beta抗体[C18C4]
    参阅全部 Insulin Receptor beta 一抗
  • 描述
    小鼠单克隆抗体[C18C4] to Insulin Receptor beta
  • 经测试应用适用于: Flow Cyt, IHC-P, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Sheep, Rabbit, Hamster, Cow, Human, Pig
  • 免疫原

    Recombinant cytoplasmic domain of human Insulin Receptor beta

  • 阳性对照
    • MCF-7, Rat-2 and L-929 cells



Our Abpromise guarantee covers the use of ab69508 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 95 kDa (predicted molecular weight: 95 kDa). (developed by ECL)


  • 相关性Insulin receptor mediates the biological activities of insulin by regulating multiple signaling pathways through activation of a series of phosphorylation cascades. The human insulin receptor is a heterotetrameric membrane glycoprotein consisting of disulfide-linked subunits in a ß-a-a-ß configuration. The ß-subunit (95kDa) possesses a single transmembrane domain with tyrosine kinase acivity, whereas the a-subunit (135kDa) is completely extracellular. The alpha subunits each contain insulin binding sites and are entirely extracellular in localization. The beta subunits each possess an extracellular domain, a single transmembrane domain, and a cytoplasmic tyrosine kinase domain. Binding of insulin to the alpha subunits induces a conformation change in the receptor which activates the kinase domain, stimulating tyrosine autophosphorylation of the receptor and tyrosine phosphorylation of at least five different insulin receptor substrates designated IRS-1-4, and Shc.
  • 细胞定位Membrane; Single pass type I membrane protein.
  • 数据库链接
  • 别名
    • CD220 antibody
    • HHF5 antibody
    • HIR B antibody
    • INSR antibody
    • Insulin receptor antibody
    • Insulin receptor subunit beta antibody
    • IR antibody
    see all

Anti-Insulin Receptor beta antibody [C18C4] 图像

  • All lanes : Anti-Insulin Receptor beta antibody [C18C4] (ab69508) at 1 µg/ml

    Lane 1 : MW marker
    Lane 2 : MCF-7 cells
    Lane 3 : Rat-2 cells
    Lane 4 : L-929 cells

    developed using the ECL technique

    Predicted band size : 95 kDa
    Observed band size : ~95 kDa (why is the actual band size different from the predicted?)
  • IHC image of ab69508 staining in human normal kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab69508, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Overlay histogram showing HepG2 cells stained with ab69508 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab69508, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

Anti-Insulin Receptor beta antibody [C18C4] (ab69508)参考文献

This product has been referenced in:
  • Wu C  et al. RSPO2-LGR5 signaling has tumour-suppressive activity in colorectal cancer. Nat Commun 5:3149 (2014). Human . Read more (PubMed: 24476626) »
  • Drapeau N  et al. Expression of SHP-1 induced by hyperglycemia prevents insulin actions in podocytes. Am J Physiol Endocrinol Metab 304:E1188-98 (2013). Read more (PubMed: 23531619) »

See all 2 Publications for this product

Product Wall

According to the Uniprot homepage the alpha chain is located from amino acid 28-758, whereas the beta chain is from aa 763-1382:

ab69508 has its immunogen in the cytoplasmic domain of ...

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I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

I am at...

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Thank you for your enquiry. I am sorry to hear this vial of ab52167 is giving you trouble. We don't have a specificIHC-P protocol available for this antibody. But If you could, please complete the formbelow so I can get an idea of your protocol and dat...

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Merci de votre intérêt pour les anticorps Abcam. Oui, je recommanderais ab37796 pour le marquage de la glucokinase et ab69508 pour le récepteur insuline beta. Par contre, pour le marquage de PCK1 je recommanderais plutôt un anticorps test...

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