Anti-Insulin Receptor alpha抗体[83-7] (Biotin) (ab79211)


  • 产品名称Anti-Insulin Receptor alpha抗体[83-7] (Biotin)
    参阅全部 Insulin Receptor alpha 一抗
  • 描述
    小鼠单克隆抗体[83-7] to Insulin Receptor alpha (Biotin)
  • 偶联物Biotin
  • 经测试应用适用于: Functional Studies, IHC-P, Flow Cytmore details
  • 种属反应性
    与反应: Sheep, Rabbit, Cow, Human, Pig
    不与反应: Mouse, Rat
  • 免疫原

    IM9 lymphocytes followed by IR.

  • 表位Exon 3
  • 阳性对照
    • IM9 lymphocyte cells. Placenta tissue.




Our Abpromise guarantee covers the use of ab79211 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Functional Studies
Flow Cyt
  • 应用说明Flow Cyt: Use at an assay dependent dilution.
    Tyrosine Kinase Assay: Use at an assay dependent dilution.
    IHC-P: Use at a concentration of 2 - 4 µg/ml for 30 minutes at room temperature.
    Staining of formalin/paraffin tissues requires digestion of tissue sections with pepsin at 1mg/ml Tris HCl, pH 2.0 for 15 minutes at room temperature or 10 minutes at 37 °C

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 相关性The human insulin receptor is a heterotetrameric membrane glycoprotein consisting of disulfide linked subunits in a beta-alpha-alpha-beta configuration. The beta subunit (95 kDa) possesses a single transmembrane domain, whereas the alpha subunit (135 kDa) is completely extracellular. The insulin receptor exhibits receptor tyrosine kinase (RTK) activity. RTKs are single pass transmembrane receptors that possess intrinsic cytoplasmic enzymatic activity, catalyzing the transfer of the gamma phosphate of ATP to tyrosine residues in protein substrates. RTKs are essential components of signal transduction pathways that affect cell proliferation, differentiation, migration and metabolism. Included in this large protein family are the insulin receptor and the receptors for growth factors such as epidermal growth factor, fibroblast growth factor and vascular endothelial growth factor. Receptor activation occurs through ligand binding, which facilitates receptor dimerization and autophosphorylation of specific tyrosine residues in the cytoplasmic portion. The interaction of insulin with the alpha subunit of the insulin receptor activates the protein tyrosine kinase of the beta subunit, which then undergoes an autophosphorylation that increases its tyrosine kinase activity. Three adapter proteins, IRS1, IRS2 and Shc, become phosphorylated on tyrosine residues following insulin receptor activation. These three phosphorylated proteins then interact with SH2 domain containing signaling proteins.
    • 细胞定位Membrane; single pass type I membrane protein.
    • 数据库链接
    • 别名
      • CD220 antibody
      • HHF5 antibody
      • HIR A antibody
      • INSR antibody
      • Insulin receptor antibody
      • Insulin receptor subunit alpha antibody
      • IR antibody
      see all

    Anti-Insulin Receptor alpha antibody [83-7] (Biotin) (ab79211)参考文献

    This product has been referenced in:
    • Macaulay SL  et al. Mutagenic structure/function analysis of the cytoplasmic cysteines of the insulin receptor. Biochem J 306 ( Pt 3):811-20 (1995). Flow Cyt . Read more (PubMed: 7702578) »
    • Soos MA  et al. Monoclonal antibodies reacting with multiple epitopes on the human insulin receptor. Biochem J 235:199-208 (1986). Human, Cow, Pig, Rabbit, Sheep . Read more (PubMed: 2427071) »

    See all 2 Publications for this product

    Product Wall

    Thank you for contacting Abcam about ab79211.
    Please find the answers to your questions below:
    The product’s clone origin is Swiss Prot #P06213
    What part of the protein would th...

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    Thank you for your inquiry.

    The epitope for this product is Exon 3. As I am not familar to what region this exon translates to, I have contacted the lab for more assistance with your inquiry. I will let you know as soon as they get back to m...

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    Thank you for contacting us.

    The originator of this product has reported that this antibody does not significantly inhibit Insulin binding as per the publication bySoos MA, et. al. Biochem J, 1986, 235(1):199-208.

    I hope this ...

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