Anti-Insulin + Proinsulin抗体[D6C4] (ab8304)

概述

  • 产品名称Anti-Insulin + Proinsulin抗体[D6C4]
    参阅全部 Insulin + Proinsulin 一抗
  • 描述
    小鼠单克隆抗体[D6C4] to Insulin + Proinsulin
  • 特异性This antibody is specific for insulin and proinsulin.
  • 经测试应用适用于: Electron Microscopy, IP, ELISA, IHC-Fr, ICC/IF, Sandwich ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Cow, Human, Pig
  • 免疫原

    Human insulin.

  • 常规说明

    Kd for this antibody is 8.1 x 10-8M.

    Detection of both insulin and proinsulin.

性能

应用

Our Abpromise guarantee covers the use of ab8304 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Electron Microscopy Use at an assay dependent concentration. PubMed: 19628478
IP 1/100.
ELISA Use a concentration of 10 µg/ml.
IHC-Fr Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration. PubMed: 24614166
Sandwich ELISA Use a concentration of 10 µg/ml. Can be paired for Sandwich ELISA with Mouse monoclonal [D3E7] to Insulin + Proinsulin (Biotin) (ab20756) and Mouse monoclonal [D3E7] to Insulin + Proinsulin (HRP) (ab28063). Use at a concentration of 10 µg/ml, 50µL per well. Can be used as Capture antibody with recommended pair(s).
IHC-P 1/1250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

靶标

Anti-Insulin + Proinsulin antibody [D6C4] 图像

  • ab8304 at 1/250 staining developing mouse pancreas tissue sections (E14/15) by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in Tris buffer was performed. The tissue was then blocked and incubated with the antibody for 2 hours. A biotinylated goat anti-mouse IgG antibody was used as the secondary.

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  • ab8304 used on human pancreas. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in Tris buffer was performed. The tissue was then blocked and incubated with the antibody for 2 hours. A biotinylated goat anti-mouse IgG antibody was used as the secondary.

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Anti-Insulin + Proinsulin antibody [D6C4] (ab8304)参考文献

This product has been referenced in:
  • Tripathi H  et al. Long-term preservation of donor corneas in glycerol for keratoplasty: exploring new protocols. Br J Ophthalmol 100:284-90 (2016). Read more (PubMed: 26508778) »
  • Ebrahimie M  et al. Efficient and simple production of insulin-producing cells from embryonal carcinoma stem cells using mouse neonate pancreas extract, as a natural inducer. PLoS One 9:e90885 (2014). ICC/IF ; Mouse . Read more (PubMed: 24614166) »

See all 5 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (pancreatic islet cells)
Loading amount 50 µg
Specification pancreatic islet cells
Gel Running Conditions Non-reduced Denaturing (4-12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Sarah Wang

Verified customer

提交于 May 15 2013



Les anticorps utilisés dans le kit ELISA ab100578 sont les anticorps ab8304 comme anticorps de capture et ab20756 comme anticorps de détection. Ces deux anticorps sont testés et garantis chez le rat. Le kit lui-même...

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I am very pleased to hear you would like to use ab8304 in WB and send us an image of your results.

This code will give you $367 off your next order before the expiration date. To redeem this offer, please submit an Abreview with the image fo...

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Thank you for contacting us. I found in our records a note stating that western blotting was added to the list of tested applications for the insulin/pro-insulin antibody ab8304 based on a single report from a customer. Unfortunately, the customer did ...

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Thank you for your enquiry.



Please find enclosed the requested documents as attachments.



If you need any further assistance in the future, please do not hesitate to contact me.

Thank you for your inquiry. 
The antibody pair that we use in the Human Insulin ELISA Kit (ab100578) is capture antibody (ab8304) and detection antibody (ab20756). 
http://www.abcam.com/insulin--proinsulin-antibody-d6c4-ab8304.html
http:/...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample Mouse Cell lysate - whole cell (MIN6 cell line)
Total protein in input 600 µg
Specification MIN6 cell line
Treatment RIPA buffer with SDS for 5 min
Immuno-precipitation step Protein A
Username

Yuichi Tsuchiya

Verified customer

提交于 Jul 05 2012

Thank you for your patience. My colleague has just added the biotinylated form of this clone to our catalog as ab133958. I hope this helps, please let me know if you need any additional assistance.

Thank you for contacting us.

While we do not currently have a biotin conjugated form of ab8304 in our catalog, I am looking into the possibility of adding one for you. I will let you know as soon as I have any information about whether this w...

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Thank you for your call yesterday and for your interest in ab8304.

I have heard back from the lab, and unfortunately there are no additional IHC images available for this antibody. This is tested in IHC-Fr in an outside lab, but we do fully g...

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