使用敲除细胞株进行验证RabMAb

Anti-Insulin degrading enzyme / IDE抗体[EPR6099] (ab109538)

概述

性能

应用

Our Abpromise guarantee covers the use of ab109538 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/10000 - 1/50000. Predicted molecular weight: 118 kDa.
ICC 1/50 - 1/100.
  • 应用说明Is unsuitable for Flow Cyt,IHC-P or IP.
  • 靶标

    • 功能Plays a role in the cellular breakdown of insulin, IAPP, glucagon, bradykinin, kallidin and other peptides, and thereby plays a role in intercellular peptide signaling. Degrades amyloid formed by APP and IAPP. May play a role in the degradation and clearance of naturally secreted amyloid beta-protein by neurons and microglia.
    • 序列相似性Belongs to the peptidase M16 family.
    • 翻译后修饰The N-terminus is blocked.
    • 细胞定位Cytoplasm. Cell surface. Present at the cell surface of neuron cells. The membrane-associated isoform is approximately 5 kDa larger than the known cytosolic isoform.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Abeta-degrading protease antibody
      • FLJ35968 antibody
      • Ide antibody
      • IDE_HUMAN antibody
      • Insulin protease antibody
      • Insulin-degrading enzyme antibody
      • Insulinase antibody
      • Insulysin antibody
      • OTTHUMP00000020097 antibody
      see all

    Anti-Insulin degrading enzyme / IDE antibody [EPR6099] 图像



    • Predicted band size : 118 kDa

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: IDE knockout HAP1 cell lysate (20 µg)

      Lane 3: K562 cell lysate (20 µg)
      Lane 4: HepG2 cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab109538 observed at 118 kDa. Red - loading control, ab8245, observed at 37 kDa.
      ab109538 was shown to specifically react with IDE when IDE knockout samples were used. Wild-type and IDE knockout samples were subjected to SDS-PAGE. ab109538 and ab8245 (loading control to GAPDH) were both diluted 1/2000 and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

    • All lanes : Anti-Insulin degrading enzyme / IDE antibody [EPR6099] (ab109538) at 1/10000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : A375 cell lysate
      Lane 4 : K562 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP-labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 118 kDa

    Anti-Insulin degrading enzyme / IDE antibody [EPR6099] (ab109538)参考文献

    ab109538 has not yet been referenced specifically in any publications.

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