Anti-Insulin degrading enzyme / IDE抗体(ab63945)

概述

性能

应用

Our Abpromise guarantee covers the use of ab63945 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 118 kDa (predicted molecular weight: 118 kDa).
ELISA 1/32000.

靶标

  • 功能Plays a role in the cellular breakdown of insulin, IAPP, glucagon, bradykinin, kallidin and other peptides, and thereby plays a role in intercellular peptide signaling. Degrades amyloid formed by APP and IAPP. May play a role in the degradation and clearance of naturally secreted amyloid beta-protein by neurons and microglia.
  • 序列相似性Belongs to the peptidase M16 family.
  • 翻译后修饰The N-terminus is blocked.
  • 细胞定位Cytoplasm. Cell surface. Present at the cell surface of neuron cells. The membrane-associated isoform is approximately 5 kDa larger than the known cytosolic isoform.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Abeta-degrading protease antibody
    • FLJ35968 antibody
    • Ide antibody
    • IDE_HUMAN antibody
    • Insulin protease antibody
    • Insulin-degrading enzyme antibody
    • Insulinase antibody
    • Insulysin antibody
    • OTTHUMP00000020097 antibody
    see all

Anti-Insulin degrading enzyme / IDE antibody 图像

  • Anti-Insulin degrading enzyme / IDE antibody (ab63945) at 0.1 µg/ml (1 hour) + K562 cell lysate in RIPA buffer at 35 µg
    Developed using the ECL technique

    Predicted band size : 118 kDa
    Observed band size : 118 kDa

Anti-Insulin degrading enzyme / IDE antibody (ab63945)参考文献

ab63945 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (mouse embryonic fibroblasts transfected with human)
Loading amount 20 µg
Specification mouse embryonic fibroblasts transfected with human
Gel Running Conditions Reduced Denaturing (8%)
Blocking step BSA as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Dr. Gillian Hunter

Verified customer

提交于 Jul 20 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"