Anti-Insulin degrading enzyme / IDE抗体(ab63137)

概述

  • 产品名称Anti-Insulin degrading enzyme / IDE抗体
    参阅全部 Insulin degrading enzyme / IDE 一抗
  • 描述
    兔多克隆抗体to Insulin degrading enzyme / IDE
  • 经测试应用适用于: ICC/IF, WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    KLH conjugated synthetic peptide selected from the Center region of human Insulin degrading enzyme/ IDE.

  • 阳性对照
    • A375 cell line lysates and human hepatocarcinoma tissue.

性能

应用

Our Abpromise guarantee covers the use of ab63137 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
WB 1/50 - 1/100. Detects a band of approximately 118 kDa (predicted molecular weight: 118 kDa).
ELISA 1/1000.
IHC-P 1/50.

靶标

  • 功能Plays a role in the cellular breakdown of insulin, IAPP, glucagon, bradykinin, kallidin and other peptides, and thereby plays a role in intercellular peptide signaling. Degrades amyloid formed by APP and IAPP. May play a role in the degradation and clearance of naturally secreted amyloid beta-protein by neurons and microglia.
  • 序列相似性Belongs to the peptidase M16 family.
  • 翻译后修饰The N-terminus is blocked.
  • 细胞定位Cytoplasm. Cell surface. Present at the cell surface of neuron cells. The membrane-associated isoform is approximately 5 kDa larger than the known cytosolic isoform.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Abeta-degrading protease antibody
    • FLJ35968 antibody
    • Ide antibody
    • IDE_HUMAN antibody
    • Insulin protease antibody
    • Insulin-degrading enzyme antibody
    • Insulinase antibody
    • Insulysin antibody
    • OTTHUMP00000020097 antibody
    see all

Anti-Insulin degrading enzyme / IDE antibody 图像

  • Anti-Insulin degrading enzyme / IDE antibody (ab63137) at 1/60 dilution + A375 cell line lysates at 35 µg

    Predicted band size : 118 kDa
    Observed band size : 118 kDa
    Additional bands at : 65 kDa. We are unsure as to the identity of these extra bands.
  • Immunohistochemical analysis of Insulin degrading enzyme / IDE expression in paraffin embedded formalin fixed human hepatocarcinoma tissue using 1/50 ab63137, followed by peroxidase-conjugated secondary antibody step and DAB staining.
  • ICC/IF image of ab63137 stained HepG2 cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab63137, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Insulin degrading enzyme / IDE antibody (ab63137)参考文献

ab63137 has not yet been referenced specifically in any publications.

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