Anti-Insulin degrading enzyme / IDE抗体(ab28561)

概述

  • 产品名称Anti-Insulin degrading enzyme / IDE抗体
    参阅全部 Insulin degrading enzyme / IDE 一抗
  • 描述
    兔多克隆抗体to Insulin degrading enzyme / IDE
  • 特异性ab28561 recognises the inactive metalloproteinase domain of IDE.
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic peptide based on the inactive metalloproteinase domain of human Insulin degrading enzyme/ IDE. (Peptide available as ab41259.)

性能

应用

Our Abpromise guarantee covers the use of ab28561 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent dilution. Predicted molecular weight: 118 kDa. A recommended starting concentration for Western blots is 1/1000 when using colorimetric substrates such as BCIP/NBT and 1/5000 for chemiluminescent substrates.

靶标

  • 功能Plays a role in the cellular breakdown of insulin, IAPP, glucagon, bradykinin, kallidin and other peptides, and thereby plays a role in intercellular peptide signaling. Degrades amyloid formed by APP and IAPP. May play a role in the degradation and clearance of naturally secreted amyloid beta-protein by neurons and microglia.
  • 序列相似性Belongs to the peptidase M16 family.
  • 翻译后修饰The N-terminus is blocked.
  • 细胞定位Cytoplasm. Cell surface. Present at the cell surface of neuron cells. The membrane-associated isoform is approximately 5 kDa larger than the known cytosolic isoform.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Abeta-degrading protease antibody
    • FLJ35968 antibody
    • Ide antibody
    • IDE_HUMAN antibody
    • Insulin protease antibody
    • Insulin-degrading enzyme antibody
    • Insulinase antibody
    • Insulysin antibody
    • OTTHUMP00000020097 antibody
    see all

Anti-Insulin degrading enzyme / IDE antibody 图像

  • All lanes : Anti-Insulin degrading enzyme / IDE antibody (ab28561) at 1/1000 dilution

    Lane 1 : Insulysin at 0.05 µg
    Lane 2 : Insulysin at 0.01 µg
    Lane 3 : Insulysin at 0.001 µg


    Predicted band size : 118 kDa
    Observed band size : 110 kDa (why is the actual band size different from the predicted?)

Anti-Insulin degrading enzyme / IDE antibody (ab28561)参考文献

This product has been referenced in:
  • Miners JS  et al. Immunocapture-based fluorometric assay for the measurement of insulin-degrading enzyme activity in brain tissue homogenates. J Neurosci Methods 169:177-81 (2008). ELISA ; Human . Read more (PubMed: 18221786) »

See 1 Publication for this product

Product Wall

There are currently no Abreviews or Questions for ab28561.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"