Anti-Insulin抗体[K36aC10] (ab6995)


  • 产品名称Anti-Insulin抗体[K36aC10]
    参阅全部 Insulin 一抗
  • 描述
    小鼠单克隆抗体[K36aC10] to Insulin
  • 特异性The antibody exhibits cross-reactivity with human proinsulin. This antibody recognizes purified insulin from the pancreas of human, bovine, horse, sheep, and proinsulin from human. Cross reaction has been observed with insulin containing cells in fixed sections of pancreas from human, porcine, dog, rabbit, bovine, sheep, rat, guinea pig and cat.
  • 经测试应用适用于: ELISA, Dot blot, IHC-P, RIAmore details
  • 种属反应性
    与反应: Mouse, Rat, Sheep, Rabbit, Horse, Guinea pig, Cow, Dog, Human, Pig
  • 免疫原

    Human insulin.

  • 阳性对照
    • Pancreas



Our Abpromise guarantee covers the use of ab6995 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent concentration. PubMed: 19572802
Dot blot Use at an assay dependent concentration.
IHC-P 1/1000. We suggest an incubation period of 30 minutes at room temperature.
RIA Use at an assay dependent concentration.


  • 功能Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
  • 疾病相关Defects in INS are the cause of familial hyperproinsulinemia (FHPRI) [MIM:176730].
    Defects in INS are a cause of diabetes mellitus insulin-dependent type 2 (IDDM2) [MIM:125852]. IDDM2 is a multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels.
    Defects in INS are a cause of diabetes mellitus permanent neonatal (PNDM) [MIM:606176]. PNDM is a rare form of diabetes distinct from childhood-onset autoimmune diabetes mellitus type 1. It is characterized by insulin-requiring hyperglycemia that is diagnosed within the first months of life. Permanent neonatal diabetes requires lifelong therapy.
    Defects in INS are a cause of maturity-onset diabetes of the young type 10 (MODY10) [MIM:613370]. MODY10 is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease.
  • 序列相似性Belongs to the insulin family.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • IDDM antibody
    • IDDM1 antibody
    • IDDM2 antibody
    • ILPR antibody
    • ins antibody
    • INS_HUMAN antibody
    • Insulin A chain antibody
    • Insulin B chain antibody
    • IRDN antibody
    • MODY10 antibody
    • Preproinsulin antibody
    • Proinsulin antibody
    • Proinsulin precursor antibody
    see all

Anti-Insulin antibody [K36aC10] 图像

  • ab6995 staining Insulin in Mouse pancreas tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 10% serum for 45 minutes at 25°C; antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/1000 in PBS) for 1 hour at 37°C. An Alexa Fluor® 594-conjugated Goat anti-mouse IgG polyclonal (1/400) (ab150116) was used as the secondary antibody.

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  • ab6995 at 1/1000 staining rat pancreas tissue sections by IHC-P. The tissue was formaldehyde fixed and blocked with BSA before incubation with the antibody for 16 hours at 4°C. An HRP conjugated goat anti-mouse antibody was used as the secondary.

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Anti-Insulin antibody [K36aC10] (ab6995)参考文献

This product has been referenced in:
  • Guo TL  et al. Genistein Protects Female Nonobese Diabetic Mice from Developing Type 1 Diabetes When Fed a Soy- and Alfalfa-free Diet. Toxicol Pathol N/A:N/A (2014). Mouse . Read more (PubMed: 24713318) »
  • Moffett RC  et al. Incretin Receptor Null Mice Reveal Key Role of GLP-1 but Not GIP in Pancreatic Beta Cell Adaptation to Pregnancy. PLoS One 9:e96863 (2014). IHC-P ; Mouse . Read more (PubMed: 24927416) »

See all 10 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (pancreas)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buffer 20min 95C
Specification pancreas
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 22°C
Fixative Formaldehyde

Dr. Vadivel Parthsarathy

Verified customer

提交于 Aug 10 2015

Application ELISA
Sample Mouse Purified protein (Pancreas)
Specification Pancreas
Type Sandwich (Detection)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.25% · Temperature: 21°C

Ms. Vruti Patel

Verified customer

提交于 Nov 18 2014

Application ELISA
Sample Human Recombinant protein (expressed in yeast)
Specification expressed in yeast
Type Sandwich (Capture)
Blocking step BSA as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Ms. Ana Chainz

Verified customer

提交于 Aug 27 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step Heat mediated
Sample Mouse Tissue sections (Pancreas)
Specification Pancreas
Blocking step Serum as blocking agent for 45 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative Paraformaldehyde

Dr. Srividya Vasu

Verified customer

提交于 Jun 06 2013

Unpurified antibodies such as ab6995 will not have a concentration stated on the datasheet.
An antibody concentration estimate of ascites fluid is 5 to 10 mg/ml.

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As your company is located in China, please contact our official distributor or our Hong Kong office to answer this questions:

I hope this information is helpful to you. Pleas...

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This antibody has been successfully tested in IHC-P and guinea pig and is theref...

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Thank you for your inquiry.

We do not have specific starting dilutions for ab6995 in ELISA and Dot Blot.

These applications were added from publications that unfortunately do not exactly describe the dilution that were used.
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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (pancreas)
Specification pancreas
Fixative Formaldehyde
Antigen retrieval step None
Permeabilization No
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C

Abcam user community

Verified customer

提交于 Nov 01 2007

Thank you for your enquiry. Ab6995 has been specifically tested in cat and shown to cross-react. I hope this information helps. Please do not hesitate to contact us if you need anything further.

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