Anti-Insulin抗体[E2E3] (ab9569)

概述

  • 产品名称Anti-Insulin抗体[E2E3]
    参阅全部 Insulin 一抗
  • 描述
    小鼠单克隆抗体[E2E3] to Insulin
  • 特异性The antibody recognizes the biologically most active forms of insulin on the C terminal end. It does not react with pro-insulin, C-peptide, thyroglobulin, albumin and insulin from rat, rabbit and/or guinea-pig (evaluated by simple-binding ELISA). The antibody labels the cytoplasm of beta cells in pancreatic islands and insulinomas (tested on formalin-fixed, paraffin-embedded tissue sections using the Streptavidin-biotinylated peroxidase method).
  • 经测试应用适用于: IHC-P, WB, ELISAmore details
  • 种属反应性
    与反应: Cow, Human, Pig
  • 免疫原

    Full length protein corresponding to Pig Insulin.

性能

应用

Our Abpromise guarantee covers the use of ab9569 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/100. Staining of formalin-fixed tissue sections requires treating in boiling 10mM citrate buffer, pH 6.0 for 10-20 minutes. Primary may be incubated for 60 mins at RT.
WB Use at an assay dependent concentration. Used at 1/100 for 1 hr on human fusion protein in transgenic seed (see Abreview sent by Cory L. Nykiforuk for furher information).
ELISA Use at an assay dependent concentration. Recommended for insulin monitoring in sera from patients with diabetes or insulin producing tumors.

靶标

  • 功能Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
  • 疾病相关Defects in INS are the cause of familial hyperproinsulinemia (FHPRI) [MIM:176730].
    Defects in INS are a cause of diabetes mellitus insulin-dependent type 2 (IDDM2) [MIM:125852]. IDDM2 is a multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels.
    Defects in INS are a cause of diabetes mellitus permanent neonatal (PNDM) [MIM:606176]. PNDM is a rare form of diabetes distinct from childhood-onset autoimmune diabetes mellitus type 1. It is characterized by insulin-requiring hyperglycemia that is diagnosed within the first months of life. Permanent neonatal diabetes requires lifelong therapy.
    Defects in INS are a cause of maturity-onset diabetes of the young type 10 (MODY10) [MIM:613370]. MODY10 is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease.
  • 序列相似性Belongs to the insulin family.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • IDDM antibody
    • IDDM1 antibody
    • IDDM2 antibody
    • ILPR antibody
    • ins antibody
    • INS_HUMAN antibody
    • Insulin A chain antibody
    • Insulin B chain antibody
    • IRDN antibody
    • MODY10 antibody
    • Preproinsulin antibody
    • Proinsulin antibody
    • Proinsulin precursor antibody
    see all

Anti-Insulin antibody [E2E3] 图像

  • Human pancreas stained with ab9569.The antibody labels the cytoplasm of ß cells in pancreatic islands and insulinomas (tested on formalin-fixed, paraffin embedded tissue sections using the Streptavidin-biotinylated peroxidase method).

Anti-Insulin antibody [E2E3] (ab9569)参考文献

This product has been referenced in:
  • Tian M  et al. Alternative immunomodulatory strategies for xenotransplantation: CD80/CD86-CTLA4 pathway-modified immature dendritic cells promote xenograft survival. PLoS One 8:e69640 (2013). IHC-P . Read more (PubMed: 23922766) »
  • Nykiforuk CL  et al. Transgenic expression and recovery of biologically active recombinant human insulin from Arabidopsis thaliana seeds. Plant Biotechnol J 4:77-85 (2006). WB ; Human . Read more (PubMed: 17177787) »

See all 2 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Monkey Tissue sections (Pancreas)
Antigen retrieval step None
Permeabilization No
Specification Pancreas
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 20°C
Fixative Formaldehyde
Username

Dr. Nizar Mourad

Verified customer

提交于 Apr 19 2016

Thank you very much for your call today and for your enquiry.

As we discussed, please keep me updated about the results with this antibody. Should you have any trouble with it, I will be happy to set up the testing program for you in retrospe...

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Thank you for your enquiry.
The antibody should be OK. I would recommend using the antibody in an experiment using a positive control sample to make sure it is still active.
Store remainder of antibody at 4 degrees C.
I hope this is help...

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Thank you for contacting us. Because the sequence is conserved between the monomeric and hexameric forms of insulin, it would not be possible to get antibodies that would be specific for one form over the other. The easiest way to tell the difference b...

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Thank you for your inquiry and I apologize for the delay in this reply.


We do not test this antibody in WB in-house. The only information we have about the use of this antibody is from customer data in the form of an Abreview and refere...

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Thank you for your inquiry. Please excuse the delay of my answer, but I am contacting the source of ab8304 and haven't heard back from them yet. But I am happy to confirm that ab9569 will weakly detect non-reduced insulin (needed to load at least...

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According to our records, ab9569 was proving difficult to use in WB and we were in contact in order to help resolve the issue. Looking at our correspondence, it appears that we are awaiting more details in order to help us better understand the dif...

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Thank you for your enquiry. I apologize for the delay in replying to you. It has taken some time to gather all the required information. Sensitivity of antibodies in ELISA assays depends very much on the individual assay procedure and the type o...

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Thank you for your enquiry. I am sorry to hear that you have been having difficulties with this antibody. I have read your technical questionaire and you are largely following an ELISA protocol that we would recommend. There is no reason why these ...

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Application Western blot
Sample Arabidopsis thaliana Recombinant protein (total seed extract)
Specification total seed extract
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Dr. Cory L Nykiforuk

Verified customer

提交于 Feb 24 2006

1-10 of 13 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"