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Full length protein corresponding to Pig Insulin.
Our Abpromise guarantee covers the use of ab9569 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100. Staining of formalin-fixed tissue sections requires treating in boiling 10mM citrate buffer, pH 6.0 for 10-20 minutes. Primary may be incubated for 60 mins at RT.|
|WB||Use at an assay dependent concentration. Used at 1/100 for 1 hr on human fusion protein in transgenic seed (see Abreview sent by Cory L. Nykiforuk for furher information).|
|ELISA||Use at an assay dependent concentration. Recommended for insulin monitoring in sera from patients with diabetes or insulin producing tumors.|
Formalin-fixed, paraffin-embedded monkey pancreas tissue stained for Insulin using ab9569 at 1/800 dilution in immunhistochemical analysis.
Goat Ani-mouse HRP was used as the secondary antibody.