概述

  • 产品名称Anti-Insulin抗体
    参阅全部 Insulin 一抗
  • 描述
    兔多克隆抗体to Insulin
  • 经测试应用适用于: IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Cat, Dog, Pig, Chimpanzee, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Insulin.

    (Peptide available as ab86658.)

  • 阳性对照
    • This antibody gave a positive staining pattern in formalin-fixed paraffin-embedded Mouse Pancreas tissue section and Human pancreas tissue section. This antibody gave a positive result when used in the following methanol fixed cell lines: RIN-5F.

性能

应用

Our Abpromise guarantee covers the use of ab63820 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 1 µg/ml.
ICC/IF Use a concentration of 5 µg/ml.

靶标

  • 功能Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
  • 疾病相关Defects in INS are the cause of familial hyperproinsulinemia (FHPRI) [MIM:176730].
    Defects in INS are a cause of diabetes mellitus insulin-dependent type 2 (IDDM2) [MIM:125852]. IDDM2 is a multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels.
    Defects in INS are a cause of diabetes mellitus permanent neonatal (PNDM) [MIM:606176]. PNDM is a rare form of diabetes distinct from childhood-onset autoimmune diabetes mellitus type 1. It is characterized by insulin-requiring hyperglycemia that is diagnosed within the first months of life. Permanent neonatal diabetes requires lifelong therapy.
    Defects in INS are a cause of maturity-onset diabetes of the young type 10 (MODY10) [MIM:613370]. MODY10 is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease.
  • 序列相似性Belongs to the insulin family.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • IDDM antibody
    • IDDM1 antibody
    • IDDM2 antibody
    • ILPR antibody
    • ins antibody
    • INS_HUMAN antibody
    • Insulin A chain antibody
    • Insulin B chain antibody
    • IRDN antibody
    • MODY10 antibody
    • Preproinsulin antibody
    • Proinsulin antibody
    • Proinsulin precursor antibody
    see all

Anti-Insulin antibody 图像

  • ab63820 stained RIN-5F cells. The cells were 4% paraformaldehyde fixed for 10 minutes at room temperature and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab63820 at 1µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • ab63820 staining Insulin in Mouse pancreas tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). The tissue section underwent formaldehyde fixation and enzymatic epitope retrieval step. The primary antibody was used at 1µg/ml and incubated with the sample for 1 hour. An HRP-conjugated Rabbit HRP polymer system was used undiluted with the secondary antibody.

  • IHC image of ab63820 staining in human pancreas formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab63820, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anti-Insulin antibody (ab63820)参考文献

This product has been referenced in:
  • Winters NI  et al. Resident progenitors, not exogenous migratory cells, generate the majority of visceral mesothelium in organogenesis. Dev Biol 391:125-32 (2014). IHC-Fr . Read more (PubMed: 24746591) »
  • Li X  et al. Oncogenic transformation of diverse gastrointestinal tissues in primary organoid culture. Nat Med N/A:N/A (2014). IHC-P ; Mouse . Read more (PubMed: 24859528) »

See all 6 Publications for this product

Product Wall

Application ELISA
Sample Cow Serum (Cow Serum (Testing antibody ab63820 for use in RIA)
Specification Cow Serum (Testing antibody ab63820 for use in RIA
Type Direct
Username

Dr. Duane Keisler

Verified customer

提交于 Aug 22 2014

Thank you for your reply.

While we have a number of anti-Insulin antibodies which you would be able to conjugated to an organic fluorophore, we currently do not offer AlexaFluor 500 in our EasyLink conjugation kits. We do offer a number of...

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Thank you for contacting us. The antibody should recognize the B chain.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. ...

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Thank you for your phone call.
I am checking with the lab regarding an ICC/IF image for ab124466 and will let you know when I hear back.
Below is the information about the testing discount program as well as your discount code:

DISC...

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Thank you for contacting us.

I have checked our insulin antibodies, but the majority was raised against the full length insulin protein. Only ab65454 was raised against the a peptide between 30˜90 amino acids and ab63820 which was raised aga...

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Merci pour votre question.

Cet anticorps contient tous les anticorps du sérum de lapin qui ont une forte affinité contre l'immunogène. Nous n'avons pas testé l'identité des ces immunoglobulines. Ceci sont normalement les IgG. A ma connaissan...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (pancreas)
Specification pancreas
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: autoclave
Permeabilization Yes - tritonX
Blocking step Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Nov 23 2011

Thank you for contacting us. Please choose antibodies from the following catalogue numbers: anti Ghrelin ab48285, ab117620, ab15861, ab57222, ab104307 These antibodies are tested in Indirect ELISA so are guaranteed for this application and ap...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Pancreas)
Specification Pancreas
Fixative Formaldehyde
Antigen retrieval step Enzymatic
Username

Abcam user community

Verified customer

提交于 May 14 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"